山东医药2018，Vol.57Issue(7)：28-32,5.DOI:10.3969/j.issn.1002-266X.2018.07.007
鱼腥草水提液及其主要成分体外对71型肠道病毒的抑制作用观察
Inhibitory effect of water extract of Houttuynia cordata Thunb and its main ingredients on enterovirus type 71 in vitro
摘要
Abstract
Objective To observe the anti-enterovirus type 71(EV71)effects of Houttuynia cordata Thunb water ex-tract and its main ingredients,including chlorogenic acid,rutin,spasinin,and quercetin in vitro.Methods The RD cells (derived from human rhabdomyosarcoma cells)were divided into the Houttuynia cordata group,hyperoside group,querce-tin group,rutin group,chlorogenic acid group,and control group.Cells in the Houttuyniae cordata group were given water extracts of Houttuynia cordata at the concentrations of 5 000,2 500,1 250,625,and 313 mg/L;cells in the quercetin and rutin groups were added with quercetin and rutin at the concentrations of 10.00,5.00,2.50,1.25, and 0.63 mg/mL;cells in the hyperoside and chlorogenic acid groups were given hyperoside and chlorogenic acid,respectively,with the con-centrations of 4.00,2.00,1.00,0.50,and 0.25 mg/mL;cells in the control group were added with the cell culture flu-id.We observed the cytopathic effect(CPE)and calculated the inhibition rate of virus replication,half maximal inhibitory concentration(IC50),inhibition rate of virus growth,inhibition rate of virus adsorption, virus inactivation rate,and virus penetration rate.Results The inhibition rate of virus replication in the Houttuynia cordata group and quercetin group was higher than that in the other four groups, and the inhibition rate increased with the increasing concentrations(all P<0.05).The inhibition rate of virus growth in the hyperin and rutin groups was higher than that in the other four groups(all P<0.05).The inhibition rate of virus adsorption in the hyperin and Houttuynia cordata group was higher than that in the other four groups(all P<0.05).The water extract and its main components of Houttuynia cordata had no effect in inacti-vating the virus.No inhibitory effect on virus penetration was observed in each group at 15 and 30 min.At 60 min,the in-hibition rate of virus penetration was higher in houttuyniae,quercetin,rutin and chlorogenic acid groups than in the hypero-side group and control group(all P<0.05).The inhibition rate of virus penetration of the quercetin group and rutin group decreased with the decreasing drug concentrations, but increased in the chlorogenic acid group.Conclusions The water extract of Houttuynia cordata Thunb and its main components all have the effect of anti-EV71 in vitro,but had no effect in inactivating the virus.Quercetin has the effect of inhibiting virus replication.Hyperin and rutin block the growth of the vi-rus,hyperoside can inhibit virus adsorption,and quercetin,rutin,chlorogenic acid can inhibit the virus penetration.关键词
鱼腥草水提液/绿原酸/芦丁/金丝桃苷/槲皮素/肠道病毒/肠道病毒71型Key words
water extract of Houttuynia cordata Thunb/chlorogenic acid/rutin/hyperin/quercetin/enterovirus/en-terovirus type 71分类
医药卫生引用本文复制引用
张慧锋,郭淑英,马莹慧,庄艳,于欢..鱼腥草水提液及其主要成分体外对71型肠道病毒的抑制作用观察[J].山东医药,2018,57(7):28-32,5.基金项目
吉林省教育厅"十三五"科学技术研究项目(2015-379) （2015-379）
吉林医药学院创新创业项目(2016049). （2016049）
