山东医药2018，Vol.58Issue(12)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.12.001
广泛期与局限期小细胞肺癌差异表达miRNAs的筛选及生物信息学分析
Screening and bioinformatics analysis of differentially expressed miRNAs between extensive-stage SCLC and limited-stage SCLC
摘要
Abstract
Objective To screen out the differentially expressed miRNAs between extensive-stage and limited-stage small cell lung cancer(SCLC)and to analyze the function of the targeting gene of differentially expressed miRNAs. Methods The microarray data related to SCLC metastasis were downloaded from the Gene Expression Omnibus(GEO). Qlucore Omics Explorer3.0 software was used to screen out differentially expressed miRNAs between extensive -stage(ED group)and limited-stage SCLC(LD group).Bioinformatics method was used to predict the target genes of differential miR-NAs,and the target genes were analyzed with GO enrichment analysis,KEGG pathway analysis,and STRING protein inter-action analysis.Results There were 17 differentially expressed miRNAs(P<0.05, |Fold change|≥2)related to SCLC metastasis between ED and LD groups.The bioinformatics results showed that these target genes complementarily binding to miRNAs were enriched in the endocytosis pathway,Ras pathway,Rap1 pathway,PI3K-Akt signaling pathways,Focal ad-hesion,in which,the key target genes of STAT3,SMAD3,E2F1,MMP2,SP1,AR,IGF1R,NFKB1 and AKT2 played a core role in the interaction network.Conclusion Seventeen differentially expressed miRNAs are found between the ex-tensive-stage and limited-stage SCLC,and the miRNAs may regulate the Ras,Rap1,PI3K-Akt,and other signaling path-ways and thus regulate the down-stream target proteins which are involved in the process of SCLC metastasis.关键词
小细胞肺癌/肿瘤转移/微小RNA/生物信息学/基因芯片Key words
small cell lung cancer/neoplasm metastasis/microRNAs/bioinformatic/gene microarray分类
医药卫生引用本文复制引用
薛亚军,耿涛,黄冰,罗波,魏育涛..广泛期与局限期小细胞肺癌差异表达miRNAs的筛选及生物信息学分析[J].山东医药,2018,58(12):1-4,4.基金项目
国家自然科学基金资助项目(81460059) （81460059）
兵团博士基金资助项目(2014BB019). （2014BB019）
