山东医药2018，Vol.58Issue(12)：18-21,4.DOI:10.3969/j.issn.1002-266X.2018.12.005
膀胱癌组织某一特异性EST片段全长扩增及生物信息学分析
Amplification and bioinformatics analysis of a specific EST fragment of bladder cancer tissues
摘要
Abstract
Objective To amplify the total length of a specific expressed sequence tag(EST)fragment of bladder cancer and to perform the bioinformatics analysis on it.Methods We collected 1 case of bladder cancer tissues which were pathologically confirmed bladder transitional cell carcinoma.The RNA from the bladder cancer tissues was extracted, and then we obtained the 5′end DNA sequence and 3' end DNA sequence of the EST fragment by RACE,and used the GeneTool to synthesize the results of DNA sequence.We analyzed the full length sequence of cDNA and its corresponding protein with bioinformatics,and submitted it to the NCBI to get GenBank accession number and protein ID by sequin soft -ware.Results We successfully got the 5′end and 3′end of the specific EST fragment,and used the GeneTool to synthe-size a full sequence of 1839bp,and it had integrated ORF,chromosome was located in 17p13.3.We got the GenBank ac-cession number,that was KR780251 in NCBI,and the ID of polypeptide sequence acquired protein translationed by ORF was AKO62675.Conclusion The gene of bladder cancer tissue-specific EST fragment is KR780251 with a full-length se-quence of 1839 bp and contains an intact ORF.The encoded protein AKO62675 may be a mutant from the normal bladder tissues.关键词
膀胱癌/基因/表达序列标签片段/变异/KR780251/AKO62675/生物信息学Key words
urinary bladder carcinoma/gene/expressed sequence tag/mutation/KR780251/AKO62675/bioinfor-matics分类
医药卫生引用本文复制引用
赵振理,胡少华,郝川,史舒,任来成..膀胱癌组织某一特异性EST片段全长扩增及生物信息学分析[J].山东医药,2018,58(12):18-21,4.基金项目
山西省卫计委科研课题基金资助项目(201201077). （201201077）
