山东医药2018，Vol.58Issue(12)：26-29,4.DOI:10.3969/j.issn.1002-266X.2018.12.007
花生四烯酸对TNF-α/放线菌素D诱导内皮细胞凋亡的影响及作用机制
Effect of arachidonic acid on apoptosis of endothelial cells induced by TNF-αand ActD
摘要
Abstract
Objective To investigate the effect and mechanism of arachidonic acid on the apoptosis of endothelial cells induced by TNF-αand actinomycin D(ActD).Methods The human umbilical vein endothelial cells cultured in vitro were randomly divided into the blank group,model group,EET group,and EET+3MA group.The blank group was cultured normally.In the latter three groups,TNF-α(10 ng/mL)and ActD(5 ng/mL)were added to induce apoptosis, meanwhile,the EET group was added with 14,15-epoxyeicosatrienoic acids(EET)(100 nmol/L),and the EET+3MA group was added with 14 15-EET(100 nmol/L)and 3-methyl adenine(3-MA)(2 mmol/L).The apoptosis rate was de-tected by flow cytometry at 24 h.Western blotting was used to detect the microtubule-related protein light chain 3-Ⅰ(LC3-Ⅰ)and 3-Ⅱ(LC3-Ⅱ), and the LC3-Ⅱ/LC3-Ⅰ was calculated.The activity of caspase-8 and 9 was detected by ELISA.Results The apoptosis rates in the blank group,EET group,and EET+3MA group were lower than that in the model group(P<0.01), and the apoptosis rate in the EET +3MA group was higher than that in the EET group(P<0.05).The LC3-Ⅱ/LC3-Ⅰratios in the EET group and EET +3MA group were higher than those in the model group and the blank group(P<0.05 or P<0.01).The LC3-Ⅱ/LC3-Ⅰ ratio in the EET+3MA group was lower than that in the EET group(P<0.01).The activity of caspase-8 and caspase-9 in the model group, EET group,EET+3MA group was higher than that in the blank group,the model group was higher than EET +3MA group and EET group,and EET+3MA group was higher than EET group(P<0.05 or P<0.01).Conclusion Arachidonic acid can inhibit the apoptosis of en-dothelial cells induced by TNF-α,and the induced autophagy by inhibiting caspase-8 and caspase-9 may be its mechanism.关键词
人脐静脉内皮细胞/细胞凋亡/花生四烯酸/肿瘤坏死因子α/放线菌素D/微管相关蛋白轻链3/3-甲基腺嘌呤Key words
human umbilical vein endothelial cells/apoptosis/arachidonic acid/tumour necrosis factor-α/actinomy-cin D/microtubule-related protein light chain 3/3-methyl adenine分类
医药卫生引用本文复制引用
陈积雄,黄晓燕,林文婷,何扬利,符秀虹,曾敏,汪道文..花生四烯酸对TNF-α/放线菌素D诱导内皮细胞凋亡的影响及作用机制[J].山东医药,2018,58(12):26-29,4.基金项目
海南省自然科学基金资助项目(817321). （817321）
