陕西中医2018，Vol.39Issue(3)：282-285,后插1,5.DOI:10.3969/j.issn.1000-7369.2018.03.003
聚山梨酯80修饰的天麻素脂质体制备研究
Study on the preparation technology of the gastrodin liposomes modified with polysorbate 80
摘要
Abstract
Objective:To optimize the formulation of the gastrodin liposomes modified with polysorbate 80 with thin film ultrasonic method by Box-Behnken design and response surface method.Methods:The influence fac-tors included the concentration of lipid,the ratio of phosphatidylcholine to cholesterol,the ratio of lipid to drug.The evaluation parameters included entrapment efficiency,drug loading.Experiments were done on the theory of Box-Behnken design.The data were simulated and the possibly optimal formulation was predicted by response surface method.Results:The optimal formulation were as follow as the concentration of lipid was 16 mg/ml,phosphatidyl choline-cholesterol ratio of 3.05:1,lipid-drug ratio of 5:1.According to the optimal conditions,the mean diameter, polydis persity index,zeta potential of the liposome were(97.63 ± 1.53)nm,(0.27 ± 0.02)and(-14.1 ± 0.83)mv,en-trapment efficiency,drug loading were(35.17 ± 0.25)% and(7.03 ± 0.05)%,respectively.Conclusion:The prepa-ration of liposomes bythin film ultrasonic method is simple and feasible.The gastrodin liposomes modified with polysorbate 80 were pale blue.The characterization results showed that the liposomes were spherical in shape and stable in nature,which lay the foundation for further research on in vivo research.关键词
天麻素/聚山梨酯/中药Key words
Gastrodin/Polysorbate Traditional/Chinese medicine分类
医药卫生引用本文复制引用
穆珺,王春柳,张红,刘洋,马虎强,李晔..聚山梨酯80修饰的天麻素脂质体制备研究[J].陕西中医,2018,39(3):282-285,后插1,5.基金项目
陕西省科技统筹创新工程计划项目(2016KTCL03-14)陕西省创新药物研究中心资助项目(2017YWZX-02) （2016KTCL03-14）
