色谱2018，Vol.36Issue(3)：185-189,5.DOI:10.3724/SP.J.1123.2017.11045
钴酞菁功能化聚合物整体柱的制备及用于转铁蛋白糖肽的富集
Preparation of cobalt phthalocyanine functionalized polymer monolith and application to enrichment of transferrin glycopeptides
摘要
Abstract
Poly(glycidyl methacrylate-ethyleneglycol dimethacrylate)(Poly(GMA-EDMA)) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid(CoPcTc)was pre-pared. The monolith was used for transferrin(Tf)glycopeptide enrichment. By taking advan-tage of hydrogen bonds between isoindole subunits of phthalocyanine and glycans and coordina-tion interaction between cobalt and glycopeptides,the monolithic material was efficient and selective. After enrichment of transferrin through the functionalized monolith,17 glycopeptides were identified by electrospray ionization quadrupole time-of-flight mass spectrometry. When the concentration of transferrin was reduced to 8.8×10-10mol/L,three glycopeptides could still be obtained. The present method has great potential for trace sample analysis.关键词
制备/聚合物整体柱/富集/酞菁/转铁蛋白Key words
preparation/polymer monolith/enrichment/phthalocyanine/transferrin(Tf)分类
化学化工引用本文复制引用
张文娟,宋乃忠,贾琼..钴酞菁功能化聚合物整体柱的制备及用于转铁蛋白糖肽的富集[J].色谱,2018,36(3):185-189,5.基金项目
国家重点实验室开放课题(sklssm201719).Open Project of State Key Laboratory(No.sklssm201719). （sklssm201719）
