色谱2018，Vol.36Issue(3)：237-244,8.DOI:10.3724/SP.J.1123.2017.11055
纳米氧化锆沉积棉花纤维的制备及其在磷酸化多肽快速富集中的应用
Preparation of zirconia nanoparticles deposited cotton fibers and their application in the rapid enrichment of phosphopeptides
摘要
Abstract
A kind of cotton-based fibrous adsorbent,zirconia nanoparticles coated cotton fiber (ZrO2-CF),was successfully prepared by liquid-phase deposition of zirconia nanoparticles on the surface of cotton fiber. And the ZrO 2-CF fibrous adsorbent was packed in pipet tips for the enrichment of phosphopeptide by pipetting up and down within 2 min,which was simple and fast. Nine phosphopeptides were identified from the digestion of β-casein after ZrO 2-CF extrac-tion. ZrO2-CF exhibited good selectivity in the enrichment of phosphopeptides from the diges-tion mixture of β-casein and BSA(molar ratio is 1:100)in which four phosphopeptides were identified. And it also showed excellent extraction efficiency in actual biological samples such as non-fat milk and human serum in which four and nine phosphopeptides were identified, respectively.关键词
氧化锆/纳米材料/液相沉积法/磷酸化多肽/富集Key words
zirconia/nanoparticles/liquid phase deposition/phosphopeptide/enrichment分类
化学化工引用本文复制引用
余琼卫,方凯敏,何小梅,郑杰,冯钰锜..纳米氧化锆沉积棉花纤维的制备及其在磷酸化多肽快速富集中的应用[J].色谱,2018,36(3):237-244,8.基金项目
国家自然科学基金(31671929,31670373).National Natural Science Foundation of China(Nos.31671929,31670373). （31671929,31670373）
