色谱2018，Vol.36Issue(3)：245-252,8.DOI:10.3724/SP.J.1123.2017.11050
固定化β-葡萄糖醛酸酶反应器的制备及其在4-甲基亚硝胺基-1-(3-吡啶)-1-丁醇的O-糖苷化合物分析中的应用
Preparation of immobilized β-glucuronidase enzyme reactor for the analysis of O-glucuronides of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol
摘要
Abstract
An immobilized β-glucuronidase enzyme reactor(IMER)based on organic-silica gel hybridized capillary column was prepared by the sol-gel method with acrylamide(AAM)as the organic functional monomer. The effects of the amounts of tetramethyl orthosilicate(TMOS), 3-glycidoxypropyltrimethoxysilane(γ-MAPS),acrylamide(AAM),polyethylene glycol(PEG), and the water bath temperature on the quality of the microscopic structure were studied. After optimization,the monolithic column with good permeability and high mechanical strength was obtained. Then β-glucuronidase was covalently immobilized,and the hydrolysis efficiency of the β-glucuronidase IMER was investigated. As the substrate,O-glucuronides of 4-(methylnitro-samino)-1-(3-pyridyl)-1-butanol(NNAL-O-Gluc)were hydrolyzed at room temperature, indicating the high hydrolysis efficiency of the β-glucuronidase IMER,which demonstrated its high potential for the high throughput analysis of tobacco metabolites in the future.关键词
有机-硅胶杂化整体柱/β-葡萄糖醛酸酶/固定化酶反应器/4-甲基亚硝胺基-1-(3-吡啶)-1-丁醇的O-糖苷化合物Key words
organic silica hybrid monolithic column/β-glucuronidase/immobilized enzyme reactor(IMER)/O-glucuronides of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol(NNAL-O-Gluc)分类
化学化工引用本文复制引用
王婷,姚二民,邓楠,边阳阳,刘萍萍,张柯,陈千思,李斌..固定化β-葡萄糖醛酸酶反应器的制备及其在4-甲基亚硝胺基-1-(3-吡啶)-1-丁醇的O-糖苷化合物分析中的应用[J].色谱,2018,36(3):245-252,8.基金项目
国家自然科学基金项目(21505158,81600046).National Natural Science Foundation of China(Nos.21505158,81600046). （21505158,81600046）
