水生生物学报2018，Vol.42Issue(2)：271-276,6.DOI:10.7541/2018.034
基于SNP标记的短须裂腹鱼自然群体遗传多样性分析
GENETIC DIVERSITY ANALYSIS OF NATURAL POPULATION OF SCHIZOTHORAX WANGCHIACHII BASED ON SNP MARKERS
摘要
Abstract
Schizothorax wangchiachii,with high ecological and great economic value,is a rare indigenous fish of Jin-sha River.The SNP markers of Schizothorax wangchiachii were developed by SLAF-seq technology in this paper.Fur-ther analysis on genetic diversity of Schizothorax wangchiachii was also done in this paper.The results showed that 709785 SLAF labels were obtained through SLAF-seq technology, and 243304 were polymorphisms. 777082 group SNP markers were obtained through bioinformatics analysis. 598354 effective SNP markers were collected to analyze the genetic diversity of Schizothorax wangchiachii.The results showed that the observed allele number of wild popula-tion was 2.005, and the expected allele number was 1.5272. The observed heterozygosity was 0.2007, while the expec-ted heterozygosity was 0.316. The average genetic distance was 0.1523, while the genetic diversity index was 0.3386. The Shnnon Wiener index was 0.4827,and the PIC was 0.2562.The MAF was 0.2313.The figures showed that the ge-netic diversity of wild Schizothorax wangchiachii between Ahai section and Longkaikou section was in the medium level.It related to artificial disturbance.Recently,the wild Schizothorax wangchiachii numbers are decreasing.Hence, this research plays an important role in protecting the germplasm resource of Schizothorax wangchiachii.关键词
短须裂腹鱼/SNP标记/SLAF-seq/遗传多样性/自然群体Key words
Schizothorax wangchiachii/SNP marker/SLAF-seq/Genetic diversity/Natural population分类
生物科学引用本文复制引用
李光华,金方彭,周睿,武祥伟,吴俊颉,冷云,高海涛,张文魁..基于SNP标记的短须裂腹鱼自然群体遗传多样性分析[J].水生生物学报,2018,42(2):271-276,6.基金项目
云南省重大科技专项(2016ZA003) （2016ZA003）
云南省珍稀濒危土著鱼收集驯养及种质资源库建设(20151105560138)资助[Supported by the major scientific and technological project of Yunnan province(the project number is 2016ZA003) （20151105560138）
The collection and domestication of Yunnan rare and endangered indigenous fish and construction of genetic resources center(the project number is 20151105560138)] （the project number is 20151105560138）
