水生生物学报2018，Vol.42Issue(2)：307-312,6.DOI:10.7541/2018.039
中华鳖GHRL基因SNPs的筛选及生长性状的关联分析
SNPS DETECTION OF GHRL GENE AND ITS ASSOCIATION WITH GROWTH TRAITS OF CHINESE SOFT-SHELL TURTLE(PELODISCUS SINENSIS)
摘要
Abstract
To study the association between GHRL gene and growth traits of Chinese Soft-shell Turtle(Pelodiscus sin-ensis),In this study,we detected and found 14 SNPs of GHRL gene of Pelodiscus Sinensis by DNA sequencing:C289T, G501T, T738C, G776T, A841G, T885C, T2960C, A2987T, G3390A, A3857C, G4718A, T4820C, A4850C and T4979C. The genotype and gene frequency of these SNPs in 120 Chinese Soft-shell Turtle were assayed by using the method of Bio-Mass Spectrometry. The result showed that all the loci were found in Hardy-Weinberg equilibrium in the Chinese Soft-shell Turtle population. The growth traits (body weight, body length, body width, body height and calipash length) of CT and CC diplotype were significantly higher than those of TT diplotype in locus C289T (P<0.05). The growth traits (Body weight, body length, body width and calipash length) of AB diplotype were significantly higher than those of AA diplotype in locus S2 (P<0.05). The growth traits (Body length and body width) of AG diplotype were significantly higher than those of AA diplotype in locus G3390A (P<0.05). The growth traits (Body length, body width and calipash length) of AG diplotype were significantly higher than those of AA diplotype in locus G4718A (P<0.05). The results suggested that those loci identified in our study were closely related to the growth performance of Chinese Soft-shell Turtle; therefore it could demonstrating great potentials to be used as a potential molecular markers for better breeding.关键词
中华鳖/GHRL/SNP/生长相关Key words
Pelodiscus sinensis/GHRL/SNP/Growth分类
农业科技引用本文复制引用
李纯,陈辰,赵建,洪孝友,李伟,朱新平..中华鳖GHRL基因SNPs的筛选及生长性状的关联分析[J].水生生物学报,2018,42(2):307-312,6.基金项目
广东省海洋渔业科技与产业发展专项科技攻关与研发项目(A201501A01)资助[Supported by the Marine Fishery and Industrial Development Special Scientific&Technological Research and Development Projects of Guangdong Province(A201501A01)] （A201501A01）
