水生生物学报2018，Vol.42Issue(2)：349-355,7.DOI:10.7541/2018.044
转基因鲤鱼与对照鲤肠道微生物群落差异研究
COMPARISON BETWEEN THE INTESTINAL BACTERIAL COMMUNITIES OF THE TRANSGENIC COMMON CARP AND THE CONTROLS
摘要
Abstract
To investigate the intestinal bacterial communities differences between the transgenic common carp and con-trols, carp intestinal bacterial community at different developmental stages (6 day, 2 month, 5 month) were used for 454 high throughput sequencing. Detrended correspondence analysis (DCA) showed that the intestinal bacterial communi-ties of transgenic common carp and controls at 2 month age were significantly different. Alpha diversity and evenness results indicated that gut microbial diversity of transgenic common carp was higher than that of the control. We found that transgenic carp gut had more bacteria belonging to Firmicutes, and the controls had more bacteria belonging Bac-teroidetes. The Bacteroidetes/Firmicutes ratio of transgenic common carp was significantly lower than that of controls in 2 month age. All data showed that transgenic common carp had different intestinal bacterial composition compared with wild controls at three developmental stages, especially at 2 month stage. Our findings provide insight into the in-teraction and mechanism between microbes and the host.关键词
转基因鲤/高通量测序/拟杆菌/厚壁菌Key words
Transgenic common carp/454 high throughput sequencing/Bacteroides/Firmicutes分类
生物科学引用本文复制引用
饶刘瑜,李学梅,李星浩,朱文根,余育和,颜庆云..转基因鲤鱼与对照鲤肠道微生物群落差异研究[J].水生生物学报,2018,42(2):349-355,7.基金项目
国家自然科学基金(31400109和31372202)资助[Supported by the National Natural Science Foundation of China] （31400109和31372202）
