水生生物学报2018，Vol.42Issue(2)：406-415,10.DOI:10.7541/2018.052
东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究
ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING ARCHAEA AND AMMONIA-OXIDIZING BACTERIA IN THE SURFACE SEDIMENTS OF DONGHU LAKE
摘要
Abstract
Ammonia oxidation is the rate-limiting step in nitrification. Ammonia-oxidizing archaea (AOA) and ammo-nia-oxidizing bacteria (AOB) play important roles in ammonia oxidation. Sediments are important place for nitrogen circulation in lake. This study investigated the community diversity and abundance of AOA and AOB in the surface sediments of Donghu Lake and their relationships with environmental factors utilizing high-throughput sequencing and real-time quantitative PCR of ammonia monooxygenase(amoA)gene.The results showed that AOA and AOB were dominated by Nitrosopumilus and Nitrosomonas,respectively.There were significant correlations between the com-munity structure of AOA and total nitrogen (TN) in sediments. AOB were significantly related to the contents of total organic carbon (TOC) and total phosphorus (TP). Additionally, AOA abundance was higher than that of AOB in the sediments. A significantly negative correlation between AOA abundance and temperature was detected, but there was no obvious change of AOB among the different seasons. These results implied that AOA could be the dominant driver in the ammonia oxidation in Donghu Lake.关键词
氨氧化古菌/氨氧化细菌/东湖/群落结构/丰度Key words
Ammonia-oxidizing archaea/Ammonia-oxidizing bacteria/Donghu Lake/Community composition/Abundance分类
生物科学引用本文复制引用
潘彦羽,代嫣然,王飞华,梁威..东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究[J].水生生物学报,2018,42(2):406-415,10.基金项目
国家科技支撑计划课题(2015BAD13B02) （2015BAD13B02）
湖北省科技厅面上基金项目(2015CFB275) （2015CFB275）
浙江省科技厅项目(2015C32011)资助[Supported by the National Science and Technology Support Program Project(2015BAD13B02) （2015C32011）
Hubei Province Science and Technology Department General Fund Project(2015CFB275) （2015CFB275）
Zhejiang Province Science and Technology Department Project(2015C32011)] （2015C32011）
