生态学杂志2018，Vol.37Issue(2)：605-612,8.DOI:10.13292/j.1000-4890.201802.012
响应面法优化枯草芽孢杆菌NHS1产芽孢发酵培养
Optimization of fermentation medium composition by response surface methodology for the spore production of Bacillus subtilis
摘要
Abstract
Both one-factor-at-a-time method and response surface methodology were used to enhance the spore content of Bacillus subtilis NHS1 in fermentation broth.A response surface regression equation was established based on the results of one-factor-at-a-time,PlackettBurman,steepest ascent and Box-Behnken experimental designs.The optimal medium formulation,as predicted from the equation,consisted of 1.88 g · L-1 starch,6.83 g · L-1 NaCl,5.60 g · L-1 corn flour,10 g · L-1 yeast extract powder,10 g · L-1 peptone,15 g · L-1 beef extract,2 g · L-1 glucose,3 g · L-1 MgSO4.The yield of the spore reached 2.42×109cfu · mL-1,which was 1.5-fold higher than that before the optimization by using the optimized medium at 36 ℃ with shaking at a speed of 170 r · min-1 for 72 h.关键词
响应面分析法/枯草芽孢杆菌/产孢率/发酵培养基Key words
response surface analysis/Bacillus subtilis/spore production rate/fermentation medium引用本文复制引用
胡瑞萍,丁贤,李来好,李俊伟,伍文超,徐宁..响应面法优化枯草芽孢杆菌NHS1产芽孢发酵培养[J].生态学杂志,2018,37(2):605-612,8.基金项目
国家自然科学基金项目(41606137)、广东省自然科学基金项目(2015A030313855,2015A030310260)、广州市科技计划项目(201707010439)和农业部水产品质量加工重点实验室(NYJG201308)资助. （41606137）
