生物加工过程2018，Vol.16Issue(2)：57-62,6.DOI:10.3969/j.issn.1672-3678.2018.02.008
3种前处理方法提取鸡脯肉中磺胺二甲基嘧啶的方法比较
Comparison of three pretreatment methods for the extraction of sulfadimidine in chicken breast meat
摘要
Abstract
Phosphate buffer saline(PBS)method,immunomagnetic beads adsorption method,and GB 29694—2013 method were used to extract sulfadimidine in chicken breast meat.The recovery and stability of the three methods were compared.The buffer of coupling 80 μg anti-sulfadimidine monoclonal antibody to 1 mg magnetic beads was 0.01 mol/L PBS(pH 5.0).When the dosage of immunomagnetic beads was 350 μg and the time to capture sulfadimidine was 45 minutes,the recovery and the coefficient of variation (CV)of the immunomagnetic beads adsorption method were 98.8% to 102.9% and 4.7% to 6.2%, respectively.The recovery and CV of the phosphate buffer saline method were 40.5%to 78.5%and 8.7%to 10.4%,respectively while the recovery and CV of the GB 29694—2013 method were 90% to 107.1%and 8.5% to 9.8%,respectively.The phosphate buffer saline method had low recovery(90%)and poor stability although it was rapid and convenient.Both GB 29694—2013 method and immunomagnetic beads adsorption method had high and stable recovery.The immunomagnetic beads adsorption method has advantage of time-saving and has remarkable potential for enriching target.关键词
磺胺二甲基嘧啶/鸡脯肉/前处理方法/快速检测/免疫磁富集法Key words
sulfadimidine/chicken breast meat/pretreatment method/rapid detection/immunomagnetic beads adsorption method分类
轻工纺织引用本文复制引用
刘娜梅,黎丹红,邢克宇,谢国阳,张抗抗,赖卫华..3种前处理方法提取鸡脯肉中磺胺二甲基嘧啶的方法比较[J].生物加工过程,2018,16(2):57-62,6.基金项目
江西省现代农业科研协同创新专项(JXXTCX201703-1) （JXXTCX201703-1）
国家级大学生创新创业训练计划(201701055) （201701055）
