生物加工过程2018,Vol.16Issue(2):69-73,5.DOI:10.3969/j.issn.1672-3678.2018.02.010
鸡蛋中呋喃唑酮代谢物金标试纸条快速分析
Rapid detection of furazolidone metabolite in eggs by lateral flow test strip
摘要
Abstract
Based on the monoclonal antibody for furazolidone metabolite,3-amino-2-oxazolidone(AOZ), and the indirect competitive immunoassay principle,the colloidal gold test strip was developed to detect AOZ in eggs.AOZ was derived by o-nitrobenzaldehyde and then was recognized by the antibody.The lowest detection limit was 0.02 μg/kg by naked eyes and the cut-off values was 0.5 μg/kg. The strip method could be used for rapid detection of furazolidone metabolite with high sensitivity.关键词
呋喃唑酮代谢物/单克隆抗体/鸡蛋/胶体金试纸条/快速检测Key words
furazolidone metabolite/monoclonal antibody/eggs/lateral flow test strip/rapid detection分类
轻工纺织引用本文复制引用
董雪,王丽雯,袁建,伦丽丽,张勋..鸡蛋中呋喃唑酮代谢物金标试纸条快速分析[J].生物加工过程,2018,16(2):69-73,5.基金项目
2016年江苏省食品安全快速检测关键共性技术攻关专题(JS0408-B011457-170004-PY) (JS0408-B011457-170004-PY)