生物加工过程2018，Vol.16Issue(2)：74-79,6.DOI:10.3969/j.issn.1672-3678.2018.02.011
多枝状胶体金免疫层析试纸条定量检测猪尿中沙丁胺醇
Multi?branched gold nanoparticles based immunochromatographic strip for quantitative detection of salbutamol in swine urine
摘要
Abstract
Multi-branched gold nanoflowers(AuNFs)with average size of 65 nm were use as the probes to construct a novel immunochromatographic strip for quantitative detection of salbutamol(SAL)in swine urine.Anti-SAL monoclonal antibodies were used to label AuNFs through physical adsorption.SAL-BSA and anti-mouse IgG were sprayed on nitrocellulose membrane as test line and control line,respectively. The T/C ratio based quantitative method was developed for SAL detection after 12 min running strip time. The AuNFs-based strip showed a good dynamic linear range from 0.05 to 1.0 ng/mL with half maximal inhibitory concentration at 0.143 ng/mL(n=5).The regression equation for SAL quantitative detection can be presented as y=-21.4ln x+10.407(R2=0.995).The average recoveries of intra-and inter-assays ranged from 98.86% to 110.68% with the relative standard deviation less than of 10%.The proposed AuNFs-based strip and commercial SAL-ELISA kit were used to detect 50 of SAL-spiked swine urine samples.The two methods have a good agreement(R2=0.955).关键词
多枝状胶体金/免疫层析试纸条/沙丁胺醇/定量检测/食品安全Key words
multi-branched gold nanoflowers/immunochromatographic strip/salbutamol/quantitative detection/food safety分类
医药卫生引用本文复制引用
吴雨豪,刘文娟,武燕华,周耀锋,熊勇华..多枝状胶体金免疫层析试纸条定量检测猪尿中沙丁胺醇[J].生物加工过程,2018,16(2):74-79,6.基金项目
国家重点基础研究发展计划(973计划)(2013CB127804) （973计划）
