生物加工过程2018，Vol.16Issue(2)：87-92,6.DOI:10.3969/j.issn.1672-3678.2018.02.013
食品中单核细胞增生李斯特氏菌的测量不确定度评定
Uncertainty measurement of Llsterla monocytosls in foods
岳苑 1贾冰凝 1马桂娟1
作者信息
- 1. 宁夏回族自治区食品检测中心,宁夏 银川750001
- 折叠
摘要
Abstract
According to the second plate counting method based on GB 4789.30-2016"National food safety standards,Food microbiology analysis Detection of Listeria monocytogenes"and JJF 1059.1-2012"Evaluation and representation of uncertainty measurement",we analyzed sources of measurement uncertainty in plate counting of Listeria monocytogenes to establish evaluation methods for such uncertainty.By building a mathematical model T=AB/Cd,uncertainty of measurements consisted of three parts: sample preparation,sample dilution and repeated measurement process.The effect of sample's memory acquisition, sample homogeneity, medium configuration time, culture medium sterilization temperature,incubator temperature,and culture time contributed little to the uncertainty.The test result of Listeria monocytosisplate counting method was(2.9±1.2)×106CFU/g.The extended uncertainty of 10 times for the same sample was 1.2 ×106.关键词
单核细胞增生李斯特氏菌/平板计数法/测量不确定度/食品安全Key words
Listeria monocytosis/plate counting method/measure uncertainty/food safety分类
轻工纺织引用本文复制引用
岳苑,贾冰凝,马桂娟..食品中单核细胞增生李斯特氏菌的测量不确定度评定[J].生物加工过程,2018,16(2):87-92,6.
