生物加工过程2018，Vol.16Issue(2)：93-101,9.DOI:10.3969/j.issn.1672-3678.2018.02.014
时间分辨荧光免疫层析法检测大米中黄曲霉毒素B1的不确定度分析
Uncertainty analysis for detecting aflatoxin B1in rice by time?resolved fluorescence immunochromatographic
摘要
Abstract
The uncertainty for detecting aflatoxin B1(AFB1)in rice by the time-resolved fluorescence immunochromatographic assay(TRFIA)was analyzed, and the main components that affect the uncertainty were found out.Based on the instruction in CNAS-GL06:2006 Guidance on Evaluating the Uncertainty in Chemical Analysis,a mathematical model was established,the source of uncertainty component was analyzed,the quantity of uncertainty component was calculated and evaluated.The results showed that the relative standard synthetic uncertainty was 3.703%,the relative standard total uncertainty was 7.406%,and the AFB1content in rice was(5.235 2 ± 0.387 7)μg/kg(k=2).The uncertainty mainly come from the standard curve fitting,repeatability determination and sample extraction,accounting for 20.82%,23.27% and 19.35%,respectively.In the determination process,an accurate and stable standard curve must be firstly prepared,the determination times of parallel samples should be increased, and the accuracy of extraction should be raised.The uncertainty evaluation method established in this paper could be used for judging the determination results of AFB1in rice by TRFIA.关键词
时间分辨荧光免疫层析法/黄曲霉毒素B1/大米/不确定度/食品安全Key words
time-resolved fluorescence immunochromatographic/aflatoxin B1/rice/uncertainty/food safety分类
化学化工引用本文复制引用
袁毅,张一平,董曼佳,熊晓辉,严艺琳,张东升..时间分辨荧光免疫层析法检测大米中黄曲霉毒素B1的不确定度分析[J].生物加工过程,2018,16(2):93-101,9.基金项目
江苏省经信委项目—食品安全快速检测关键共性技术(JS0408-B011457-170002-PY) （JS0408-B011457-170002-PY）
江苏省农业科技自主创新资金(CX(17) 1003) （CX(17）
