陕西医学杂志2018，Vol.47Issue(4)：411-413,416,4.DOI:10.3969/j.issn.1000-7377.2018.04.001
甲氨蝶呤对类风湿性关节炎大鼠滑膜细胞凋亡基因调控的实验研究
Effect of MTX on rheumatoid arthritis ratsynovial cell apoptosis and regulation of cell apoptosis gene
摘要
Abstract
Objective:To observe the effective of M TX on RA synovial cell apoptosis and cell apoptosis gene in rats, and explore the mechanism of MTX on the disease.Methods:Rats were prepared for adjuvant arthritis models induced bacetic acid soluble type II collagen and Freund's complete adjuvant,and"MTX"as the intervention drug;The inhibitory effects on pawedana were observed.Separation of synovial cells in all groups rats and detected the expression of apoptosis relative protein by WB and FCM.Results:The result of experiment,the role of the experimental group were better than the control group(P<0.05);in both experiment,synovial cell apoptosis was increased in the model group.while Bax、Caspase-3 and Caspase-9 expression were increased,comparing with the control group and normal group,there were statis-tically significant(P<0.05).Conclusion:MTX can induce apoptosis of synovial cell,by down-regulating Bcl-2 expression and subsequently up-regulating Bax,Caspase-3 and Caspase-9 expression.关键词
关节炎,类风湿/药物疗法/甲氨蝶呤/治疗应用/细胞凋亡/滑膜/动物,实验/大鼠/@Bcl-2/@Caspase-3Key words
Arthritis,rheumatoid/drug therapy/Methotrexate/therapeutic use/Apoptotic/Synovium Animal,laboratory/Rats分类
医药卫生引用本文复制引用
周文旭,方堃,谭湘淑,佘君,梁熹,刘江,蒲丹..甲氨蝶呤对类风湿性关节炎大鼠滑膜细胞凋亡基因调控的实验研究[J].陕西医学杂志,2018,47(4):411-413,416,4.基金项目
陕西省科技厅攻关项目(2009K17-01)中央高校基本科研业务费专项科研项目(SK2016037) （2009K17-01）
