生殖医学杂志2018，Vol.27Issue(3)：209-212,4.DOI:10.3969/j.issn.1004-3845.2018.03.003
卵母细胞激活技术在辅助生殖技术中的临床应用
Clinical application of oocyte activation in assisted reproductive technology
摘要
Abstract
Objective:To observe the effect of assisted activation on the effect of mature oocytes in patients with poor fertilization or failed fertilization,and evaluate the embryonic development.Methods:Oocyte activation technology was adopted for 12 patients with a history of poor fertilization or failed fertilization after ICSI (fertilization rate ＜30％)in Reproductive Medicine Center of Shanxi Province Maternal and Child Health Hospital from October 2014 to October 2016.A total of 105 mature oocytes were collected from 12 patients and all of them were activated by calcium ionophore A23187 combined with 6-dimethylaminopurine(6-DMAP).The prokaryotic formation and embryonic development were observed after activation of oocytes.Results:The fertilization rate and cleavage rate of the activation cycle were 63.81％ (67/105)and 88.06％ (59/67),which were significantly increased compared with previous cycle [23.96％ (23/96)and 69.57％(16/23)](P＜0.05).The numbers of transplanting embryos [(3.02±2.51)vs.(1.41±0.58)] and high quality embryos [(1.70±1.36)vs.(0.31+0.52)] in the activation cycle were significantly higher than those in the previous cycles(P＜0.05).Five patients got clinical pregnancy after oocyte activation in the 12 patients with poor fertilization or failed fertilization after ICSI.Conclusions:Assisted activation technology can improve fertilization rate and the potential of embryonic development in patients with poor fertilization or failed fertilization.关键词
卵母细胞激活/受精失败/钙离子载体A23187/6-甲基氨基嘌呤(6-DMAP)Key words
Oocyte activation/Fertilization failure/Calcium ionophore A23187/6-dimethylaminopurine(6-DMAP)引用本文复制引用
陈兵兵,贾洪响,毕星宇,武学清..卵母细胞激活技术在辅助生殖技术中的临床应用[J].生殖医学杂志,2018,27(3):209-212,4.基金项目
山西省儿童医院妇幼保健院院内基金项目 （）
