外科理论与实践2018，Vol.23Issue(1)：41-47,7.DOI:10.16139/j.1007-9610.2018.01.010
Ezetimibe抑制胆固醇结石形成的实验研究
Ezetimibs prevented cholesterol gallstone formation in mice fed with lithogenic diet
王启晗 1孙海东 1蔡劬 1胡海 2韩天权 1蒋兆彦1
作者信息
- 1. 上海交通大学医学院附属瑞金医院上海消化外科研究所,上海200025
- 2. 同济大学附属东方医院胆石病中心 同济大学医学院胆石病研究所,上海201200
- 折叠
摘要
Abstract
Objective To investigate the effect of prevention on cholesterol gallstone formation by ezetimibe (Eze) in mice.Methods Thirty adult male C57BL/6 mice were randomly divided into three groups,fed with chow diet (chow group),lithogenic diet (LD group) and lithogenic diet with 5 mg/ (kg·d) ezetimibe by oral gavage (Eze group) for 8 weeks.On sacrifice,occurrence of gallstone was observed.Serum,liver,intestine and gallbladder were collected from each mouse.The serum lipids,hepatic cholesterol and biliary lipid composition were quantified by enzymatic methods.Expression of genes involved in metabolism of cholesterol in liver and intestines were measured by real-time quantitative PCR.Results No gallstone was observed in chow group.All mice formed gallstone in LD group (100％).No gallstone was formed in Eze group also.The intestinal cholesterol absorption rate in Eze group (9.29％±4.32％) significantly reduced when compared with LD group (58.62％±3.10％) and chow group (56.42％±2.67％) (P＜0.01).Serum cholesterol level and hepatic cholesterol level in LD group [(4.99±0.50) mmol/L and (22.92±2.39) mg/g] increased markedly compared with chow group [(2.87±0.06) mmol/L and (2.45±0.08) mg/g] (P＜0.05).Eze group lowered both serum cholesterol level [(1.11±0.10) mmol/L] and hepatic cholesterol level [(2.70±0.07) mg/g] compared with LD group (P＜0.05).LD group has significantly higher biliary cholesterol content [LD group (10.87±1.46) mmol/L vs chow group (3.67±0.58) mmol/L] and cholesterol saturation index (CSI) [LD group:(1.42±0.19) vs chow group:(0.59±0.02)].Biliary cholesterol content [(2.72±0.29) mmol/L] and CSI (0.57 ±0.07) in Eze group decreased markedly compared with LD group (P＜0.01).Conclusions Eze could prevent cholesterol gallstone formation in mice through inhibition of intestinal cholesterol absorption.关键词
Ezetimibe/胆固醇结石病/小肠Key words
Ezetimibe/Cholesterol gallstone disease/Intestine分类
医药卫生引用本文复制引用
王启晗,孙海东,蔡劬,胡海,韩天权,蒋兆彦..Ezetimibe抑制胆固醇结石形成的实验研究[J].外科理论与实践,2018,23(1):41-47,7.
