外科理论与实践2018，Vol.23Issue(1)：67-71,5.DOI:10.16139/j.1007-9610.2018.01.015
高通量转录组测序初步研究结肠直肠癌新辅助治疗敏感性
Preliminary research on colorectal cancer neoadjuvant therapy sensitivity through high-throughput transcriptome sequence
摘要
Abstract
Objective To screen and identify the genes associated with sensitivity of neoadjuvant therapy for the patients with colorectal cancer through high-throughput transcriptome sequence technology and bioinformatics tools by analysis of the genes which expressed differently.Methods RNA was extracted from the tumor tissue of 22 patients with colorectal cancer who received standard neoadjuvant therapy before operation.Nine cases were divided as sensitive group and 11 cases as non-sensitive group based on tumor regression grading.Strand libraries were constructed for high-throughput sequencing.The genes filtered the false discovery rate (＜0.05) were mapped to the reference genome,and assembled.The obtained transcripts were classified and annotated and the characteristics of gene were analyzed.Results The gene database associated with neoadjuvant therapy for colorectal cancer was built preliminarily.From 22 patients with colorectal cancer tissues,89 genes were found up-regulated significantly and 112 genes down-regulated.Conclusions The genes related to the sensitivity of colorectal cancer neoadjuvant therapy were identified in this study using high-throughput transcriptome sequence.关键词
结肠直肠癌/新辅助治疗/治疗敏感性/转录组测序Key words
Colorectal Cancer/Neoadjuvant therapy/Therapy sensitivity/Transcriptome sequencing分类
医药卫生引用本文复制引用
陈献则,程兮,吴浩旋,张弢,季晓频,赵任..高通量转录组测序初步研究结肠直肠癌新辅助治疗敏感性[J].外科理论与实践,2018,23(1):67-71,5.基金项目
上海市科委项目(14411950504) （14411950504）
上海申康中心市级医院临床技能与临床创新能力3年行动计划(16CR3064B) （16CR3064B）
上海交通大学医学院博士研究生创新基金(BXJ201709) （BXJ201709）
