中国组织工程研究2018，Vol.22Issue(1)：7-12,6.DOI:10.3969/j.issn.2095-4344.0403
羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架复合骨髓间充质干细胞修复关节骨软骨缺损
Effect of poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite osteochondral scaffold combined with bone marrow mesenchymal stem cells in the repair of articular osteochondral defects
摘要
Abstract
BACKGROUND: Preliminary experiments have confirmed that poly(hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx)/collagen composite osteochondral scaffold exhibits desirable pore structure and biocompatibility. OBJECTIVE: To investigate the effect of PHBHOx/collagen composite osteochondral scaffold carrying bone marrow mesenchymal stem cells in the repair of articular osteochondral defects in the weight-bearing area of rabbits. METHODS: Cone-shaped osteochondral defects were created in the femoral medial condyle of the right knee of 30 New Zealand white rabbits. Then, the rabbit models were randomized into three groups and underwent implantation of PHBHOx/collagen composite osteochondral scaffold carrying bone marrow mesenchymal stem cells in the scaffold-cell group, PHBHOx/collagen composite osteochondral scaffold in the scaffold group and no intervention in the control group. At 4 and 12 weeks after surgery, animals were sacrificed for gross, Micro-CT, histological and immunohistochemical collagen II observations. RESULTS AND CONCLUSION: At 12 weeks after surgery, Micro-CT scanning results suggested that osteochondral defects were not repaired in the control group, repaired incompletely in the scaffold group with many new bone trabeculae, and repaired completely in the scaffold-cell group. Histological results showed that at 4 weeks after surgery, the defects in the control group were filled with amorphous tissues, subchondral bone formation just occurred in the scaffold group but increased in the scaffold-cell group. At 12 weeks after surgery, trabecular bone structure with no cartilage lacuna was observed in the control group; incomplete subchondral bone formation was observed in the scaffold group, and the cartilage layer was repaired by the fibrous tissues; in the scaffold-cell group, osteochondral defect repair was complete, with the emergence of tidal line, and the newborn cartilage was completely integrated with the surrounding normal tissue in addition to a similar thickness. At 12 weeks after surgery, collagen II basically did not express in the control group, weakly expressed in the scaffold group and highly expressed in the scaffold-cell group. In short, the PHBHOx/collagen composite osteochondral scaffold promotes the repair of articular osteochondral defects in the weight-bearing area.关键词
羟基丁酸-羟基辛酸共聚体/骨髓间充质干细胞/骨软骨缺损/一体化骨软骨支架/组织工程/干细胞分类
医药卫生引用本文复制引用
鉴晓东,张永红,张宇,刘来有..羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架复合骨髓间充质干细胞修复关节骨软骨缺损[J].中国组织工程研究,2018,22(1):7-12,6.基金项目
山西省回国留学人员科研资助项目(2011-109):新型多聚羟基烷酸软骨组织工程载体的研究 the Scientific Research Foundation for the Returned Overseas Chinese Scholars of Shanxi Province, No. 2011-109 （2011-109）
