中国组织工程研究2018，Vol.22Issue(1)：101-106,6.DOI:10.3969/j.issn.2095-4344.0418
负压条件下骨髓间充质干细胞向表皮细胞的诱导分化
Negative pressure promotes epidermal differentiation of bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: As a physical factor, negative pressure can promote the osteogenic differentiation and endothelial differentiation of mesenchymal stem cells. If a negative pressure exerts effects on the epidermal differentiation of mesenchymal stem cells, it will be highly important for the combination use of negative pressure and mesenchymal stem cells in wound healing. OBJECTIVE: To explore the effect of negative pressure on the epidermal differentiation of bone marrow mesenchymal stem cells. METHODS: Bone marrow mesenchymal stem cells of New Zealand white rabbits were isolated and cultured. Then, the passage 3 cells were induced for epidermal differentiation under negative pressure (-16.625 kPA, twice a day, once for 4 hours) as experimental group. Another cells induced under no negative pressure were used as control group. After induction, cell growth curve was drawn in each group, and the expression of cytokeratin 5 and cytokeratin 10 mRNA was examined by real-time PCR at 2 weeks after induction. RESULTS AND CONCLUSION: The cell growth of the experimental group was inhibited, and the mRNA expression of cytokeratin 5 and cytokeratin 10 was significantly increased compared with the control group (P < 0.05). These findings indicate that under the condition of negative pressures, the epidermal differentiation ability of bone marrow mesenchymal stem cells is increased, and in contrary, the cell proliferation is inhibited.关键词
干细胞/骨髓间充质干细胞/负压创面技术/细胞分化/细胞增殖/表皮分化/负压诱导/国家自然科学基金分类
医药卫生引用本文复制引用
赵伯文,徐强,葛权虎,李伯龙,彭心宇,张宏伟,吴向未..负压条件下骨髓间充质干细胞向表皮细胞的诱导分化[J].中国组织工程研究,2018,22(1):101-106,6.基金项目
国家自然科学基金项目(31271458) （31271458）
兵团应用基础研究计划(2016AG019) the National Natural Science Foundation of China, No. 31271458 （2016AG019）
Applied Basic Research Project of Xinjiang Production and Construction Corps, No. 2016AG019 （）
