中国组织工程研究2018，Vol.22Issue(8)：1281-1286,6.DOI:10.3969/j.issn.2095-4344.0149
DNA疫苗的作用机制
DNA vaccines: mechanisms of action
摘要
Abstract
BACKGROUND: In 1990, Wolff et al. reported that DNA was examined as a gene therapy tool, and emerged as a promising therapy after observations that simple injection of naked plasmid DNA and RNA led to profound transgene expression in vivo.DNA vaccines are recognized for harboring several distinguishing characteristics and advantages (including low cost, ease and rapidity of manufacturing, and stability) making them a method for addressing global health threats in the future. OBJECTIVE: To review the status and research progress of DNA vaccines in the view of mechanism of action: innate immune signaling from bacterial DNA, transfecting somatic cell by DNA vaccines, cross-presentation and cross-activation, transfecting antigen presenting cells by DNA vaccines, and apoptosis. METHODS: The first author retrieved the databases of PubMed and CNKI for the articles concerning DNA vaccines published between January 2000 and June 2017 using the keywords of "DNA vaccine, gene vaccine, plasmid DNA, cross-presentation, transfection, apoptosis"in English and Chinese, respectively. A total of 105 literatures were searched, and 47 eligible articles were included in accordance with the inclusion criteria. RESULTS AND CONCLUSION: The immunogenicity of DNA vaccines in humans has been limited by low expression levels of antigen, in comparison with the conventional protein vaccines in the past two decades. Studies on the mechanism of action of DNA vaccines in terms of antigen-presenting cell types able to cross-present DNA-encoded antigens, the activation of innate immune responses due to DNA itself and induction of cell apoptosis have suggested the opportunities to increase the immunogenicity of these vaccines.关键词
DNA疫苗/基因疫苗/DNA质粒/作用机制/交叉激活/组织构建/细胞凋亡分类
医药卫生引用本文复制引用
高晓佩,管晓燕,白国辉,刘建国..DNA疫苗的作用机制[J].中国组织工程研究,2018,22(8):1281-1286,6.基金项目
国家自然科学基金项目(81560186) （81560186）
贵州省科技厅社发攻关项目(黔科合SY字[2013]3043号) （黔科合SY字[2013]3043号）
贵州省高等学校特色重点实验室建设项目(黔教合KY字[2013]109号)和贵州省科技厅联合基金项目(黔科合J字LKZ[2012] 47号)the National Natural Science Foundation of China, No. 81560186 （黔教合KY字[2013]109号）
the Social Development Project of Science and Technology Department of Guizhou Province, No. SY[2013]3043 （）
the University Key Laboratory Construction Project of Guizhou Province, No. KY[2013]109 （）
the Joint Fund of Science and Technology Department of Guizhou Province, No. J-LKZ[2012]47 （）
