中国组织工程研究2018，Vol.22Issue(9)：1332-1337,6.DOI:10.3969/j.issn.2095-4344.0461
二氮嗪对低氧环境下骨髓间充质干细胞增殖活力及凋亡的影响
Diazoxide effects on proliferation and apoptosis of bone marrow mesenchymal stem cells under hypoxic conditions
摘要
Abstract
BACKGROUND: It is an urgent problem to effectively make bone marrow mesenchymal stem cells exert proper effects under hypoxic preconditioning. OBJECTIVE: To investigate the effects of diazoxide, a Mito-KATPchannel activator, on the proliferation and apoptosis of mouse BMSCs in hypoxic environment. METHODS: Mouse BMSCs were divided into four groups: blank control group, 0.16, 0.8, 4 μmol/L diazoxide groups. Cells intervened by diazoxide were cultured in a 10% O2incubator. MTT assay was performed to detect cell proliferation at 1, 2, 4, 6, 8 days after intervention, and Hoechst 33258 staining was performed to observe cell apoptosis at 14 days after intervention. RESULTS AND CONCLUSION: High homogeneity and purity but low proliferation of BMSCs was found. There was no significant difference in the activity of BMSCs among 0.16, 0.8, 4 μmol/L diazoxide groups (P > 0.05). In the blank control group, concentrated nuclei were dark blue in color and aggregated, and several round apoptotic bodies were found. In the diazoxide groups, apoptotic bodies were occasionally found, and no significant difference was found among different diazoxide groups. These findings indicate that a certain concentration of diazoxide can reduce cell apoptosis but has no effects on the proliferation of mouse BMSCs under hypoxic environment (10% O2).关键词
干细胞/骨髓间充质干细胞/低氧环境/二氮嗪/细胞活力/细胞凋亡/国家自然科学基金分类
医药卫生引用本文复制引用
李红波,林诗炜,姚江凌,卞阳阳,曲野,王雷涛,彭磊..二氮嗪对低氧环境下骨髓间充质干细胞增殖活力及凋亡的影响[J].中国组织工程研究,2018,22(9):1332-1337,6.基金项目
国家自然科学基金(81460339) （81460339）
海南省自然科学基金(30863) （30863）
海南医学院培育基金(HY2018-22) （HY2018-22）
the National Natural Science Foundation of China,No.81460339 （）
the Natural Science Foundation of Hainan Province,No.30863 （）
the Culture Fund of Hainan Medical University,No.HY2018-22 （）
