中国组织工程研究2018，Vol.22Issue(9)：1419-1424,6.DOI:10.3969/j.issn.2095-4344.0474
脑源性神经营养因子修饰人羊膜间充质干细胞移植改善老年痴呆大鼠的学习记忆能力
Effects of braln-derived neurotrophic factor-modified human amniotic membrane-derived mesenchymal stem cell transplantation on learning and memory abilities of alzheimer's disease rats
摘要
Abstract
BACKGROUND: A variety of stem cells have been found to be effective in the treatment of Alzheimer's disease in rats. However, few reports have been reported on the treatment of Alzheimer's disease rats with brain-derived neurotrophic factor (BDNF)-modified human amniotic membrane-derived mesenchymal stem cells. OBJECTIVE: To investigate the effects of BDNF-modified human amniotic membrane-derived mesenchymal stem cell transplantation on the learning and memory abilities of Alzheimer's disease rats. METHODS: Forty-eight Sprague-Dawley rats were divided into control group (no treatment), model group (Alzheimer's disease model), stem cell transplantation group (human amniotic membrane-derived mesenchymal stem cell transplantation+Alzheimer's disease model) and BDNF-modified stem cell transplantation group (BDNF-modified human amniotic membrane-derived mesenchymal stem cell transplantation+Alzheimer's disease model), 12 rats in each group. Learning and memory of model rats were determined in a trisection radiation maze and immunohistochemical staining was used to determine the number of p75 positive neurons at 2 weeks after cell transplantation. RESULTS AND CONCLUSION: The number of p75 positive neurons in the bevel zone and medial septal nucleus was ranked as follows: the model group < the stem cell transplantation group < the BDNF-modified stem cell transplantation group < the control group, and there were significant differences among groups (P < 0.05). The learning and memory abilities of the rats were ranked as follows: the model group < the stem cell transplantation group < the BDNF-modified stem cell transplantation group < the control group, and there were significant differences among groups (P < 0.05). In the BDNF-modified stem cell transplantation group, the number of learnings was negatively correlated with the number of p75 NGFR-positive neurons (P < 0.05), while the memory capacity was positively correlated with the number of p75 NGFR-positive neurons (P < 0.05). These findings reveal that human amniotic membrane-derived mesenchymal stem cell transplantation can improve learning and memory abilities of Alzheimer's disease rats, and BDNF-modified human amniotic membrane-derived mesenchymal stem cells can further improve this therapeutic effect.关键词
脑源性神经营养因子/羊膜间充质干细胞/细胞移植/老年痴呆/学习/记忆/p75NGFR/干细胞分类
医药卫生引用本文复制引用
高明龙,史少霞,张坤,张英东,李娜,余明,王永良..脑源性神经营养因子修饰人羊膜间充质干细胞移植改善老年痴呆大鼠的学习记忆能力[J].中国组织工程研究,2018,22(9):1419-1424,6.基金项目
河北省卫生厅科学研究项目(ZL20140099) （ZL20140099）
河北省卫生厅项目(20130578) （20130578）
the Scientific Research Project of Hebei Provincial Health Department,No.ZL20140099 （）
the project funded by the Hebei Provincial Health Department,No.20130578 （）
