中国组织工程研究2018，Vol.22Issue(9)：1457-1462,6.DOI:10.3969/j.issn.2095-4344.0479
转录共激活因子TAZ对人牙髓干细胞体外增殖的影响
Effects of TAZ, a transcriptional coactivator, on human dental pulp stem cell proliferation in vitro
张文 1罗士维 1谷爱玲 2何巍1
作者信息
- 1. 郑州大学第一附属医院口腔医学中心,河南省郑州市 450052
- 2. 濮阳市人民医院,河南省濮阳市 457099
- 折叠
摘要
Abstract
BACKGROUND:How to improve the proliferation of human dental pulp stem cells(hDPSCs)in vitro is of great importance in regenerative medicine. OBJECTIVE: To investigate the effect of TAZ, a transcriptional coactivator, on the proliferation of hDPSCs and its potential mechanisms. METHODS:hDPSCs were isolated from human dental pulps and cultured in vitro.The expression of TAZ in hDPSCs was detected by immunofluorescence method. Then, we knocked down the expression of TAZ in hDPSCs, and the proliferation of hDPSCs was measured by MTT and BrdU kit analysis respectively. The levels of CTGF and Cry61, which are the downstream target genes of TAZ, were detected by RT-qPCR and western blot assay. We also investigated the effect of TAZ on the levels of transforming growth factor-β (TGF-β) signaling proteins, Smad3 and Smad 4 by using western blot. RESULTS AND CONCLUSION: TAZ protein was expressed in hDPSCs. After the transfection by siTAZ for 24 hours, the mRNA and protein levels of TAZ were both significantly decreased. After the transfection by siTAZ for 24 and 48 hours, the proliferation of hDPSCs was obviously inhibited. Meanwhile, the mRNA and protein levels of CTGF and Cry61 were decreased by siTAZ transfection. The further research showed that TAZ silence also inhibited the expression of Smad3 and Smad 4, which belonged to the TGF-β signaling pathway. To conclude, TAZ may modulate the proliferation of hDPSCs through regulating the expression of CTGF and Cry61, and TGF-β-dependent signaling pathways may be involved in this regulation.关键词
人牙髓干细胞/转录共激活因子TAZ/干细胞/细胞增殖/TGF-β通路分类
医药卫生引用本文复制引用
张文,罗士维,谷爱玲,何巍..转录共激活因子TAZ对人牙髓干细胞体外增殖的影响[J].中国组织工程研究,2018,22(9):1457-1462,6.
