纤维素科学与技术2018，Vol.26Issue(1)：1-7,54,8.DOI:10.16561/j.cnki.xws.2018.01.10
细菌纤维素固定化漆酶对活性艳蓝KN-R脱色的研究
Decolorization of Reactive Brilliant Blue KN-R by Bacterial Cellulose-immobilized Laccase
摘要
Abstract
Immobilization of laccase on BC membrane and use for decolorization of reactive brilliant blue KN-R was investigated. Kinetics of the degrading of brilliant blue and influence of different factors on decolorization of the brilliant blue was studied. The results showed that degradation of the brilliant blue by immobilized laccase matched with two order kinetic equation. Adsorption of the brilliant blue on BC membrane matched with Freundlich isotherm. When the enzyme activity was 84.9 U, the decolorization efficiency was 73.5%. The best decolorization efficiency was 81.1% when the dye concentration was 50 mg/L. Decolorization of the brilliant blue by immobilized enzyme preferred acidic conditions, and the optimum pH was 5, with which the decolorization efficiency reached as high as 95.2%. Compared with free enzyme, the degradation rate of immobilized enzyme was increased by 2 times, and the reaction rate was increased by 3 times. The decolorization efficiency of the brilliant blue was reduced to 12.2% after 5 times repeated decolorization by the same immobilized enzyme. The absorption peaks in the UV visible absorption spectra of the decolorized solution of reactive brilliant blue KN-R almost disappeared completely, which indicated that dye was degraded.关键词
细菌纤维素/漆酶/活性艳蓝KN-R/脱色/降解Key words
bacterial cellulose/laccase/reactive brilliant blue KN-R/decolorization/degradation分类
生物科学引用本文复制引用
吴月红,袁海彬,洪枫,陈琳..细菌纤维素固定化漆酶对活性艳蓝KN-R脱色的研究[J].纤维素科学与技术,2018,26(1):1-7,54,8.基金项目
国家自然科学基金(51373031) （51373031）
上海市科委项目(15520720800) （15520720800）
中央高校基本科研业务费专项资金资助项目. （）
