纤维素科学与技术2018，Vol.26Issue(1)：8-15,16,9.DOI:10.16561/j.cnki.xws.2018.01.04
伴生介质漆酶的生产优化及其酶学特性研究
Production and Characterization of the Laccase with an Endogenous Mediator
摘要
Abstract
The laccase with an endogenous mediator was prepared with Pycnoporus sanguineus NFZH-1, to alleviate the cost and environmental problems of artificial mediator. This will be developed for the industrial application of laccase mediator system (LMS). In the present study, the production of the laccase with an endogenous mediator was improved by the optimizing of liquid medium and fermentation conditions. Its enzymatic characteristics were also revealed. The results showed that P. sanguineus NFZH-1 was a high-yield laccase-producing fungus. The laccase activity was increased by 91%, and reached to 26.1 U/mL by means of the above optimizing. The reaction of the laccase would like to happen at temperatures 50~85℃ and pH2.5~4.5. The laccase was found to be stable at temperatures below 60℃ and pH5.0~7.0. The thermostability of the laccase was also identified. The high yield of the thermostability laccase with an endogenous mediator will be significant for the industrial application of LMS.关键词
漆酶/血红密孔菌/伴生介质/液态培养基/发酵条件Key words
laccase/Pycnoporus sanguineus/endogenous mediator/liquid medium/fermentation condition分类
化学化工引用本文复制引用
冯年捷,傅瑜,王传槐..伴生介质漆酶的生产优化及其酶学特性研究[J].纤维素科学与技术,2018,26(1):8-15,16,9.基金项目
湖北工业大学博士科研启动基金. （）
