纤维素科学与技术2018，Vol.26Issue(1)：48-54,7.DOI:10.16561/j.cnki.xws.2018.01.07
间苯三酚法快速测定玉米芯水解液中单糖含量
Rapid Determination of Monosaccharides Content in Corncob Hydrolysis by Phloroglucinol Method
摘要
Abstract
The concentration of xylose and glucose in solution were determined simultaneously by phloroglucinol colorimetry. The optimal determination conditions, the interaction between two monosaccharides and its application were studied. The results showed that the maximum absorption peak of xylose and glucose was observed at the wavelength of 444 nm and 420 nm, respectively. Optimal determination conditions were as follows: 1 mL substrates with 5 mL coloring agent, boiling water bath for 10 min. Results showed that both monosaccharides obeyed Beer-Lambert's law at the concentrations of 20~120 μg/mL. The experiment indicated that the standard deviation of xylose and glucose were 0.8432 and 0.8430 μg/mL, RSD were 1.3967% and 1.3959%, respectively. Recovery rate was 100.82% and 100.65%, RSD were 0.32% and 0.46%, respectively. This method was simple and accurate and had high application in the rapid determination of glucose and xylose. However, the interaction between the two monosaccharides limited its application when two monosaccharides coexisted.关键词
间苯三酚-浓硫酸显色剂/双波长/木糖/葡萄糖/玉米芯Key words
pyrogallol-concentrated sulfuric acid color reagent/dual wavelength/xylose/glucose/corncob分类
化学化工引用本文复制引用
高晓峰,兰萌,杨明琰,段洁,樊成..间苯三酚法快速测定玉米芯水解液中单糖含量[J].纤维素科学与技术,2018,26(1):48-54,7.基金项目
陕西省社会发展科技攻关项目(2015SF263). （2015SF263）
