烟草科技2018，Vol.51Issue(3)：1-6,6.
烟草慢阴离子通道蛋白基因NtSLAH1的克隆及表达分析
Cloning and expression analysis of slowly activating anion channel gene NtSLAH1 from Nicotiana tabacum
摘要
Abstract
To clarify the function of NtSLAC (Slowly activating anion channel, SLAC) genes in chloride transport, a SLAH (SLAC homologue) gene was cloned from Nicotiana tabacum by homology search, named as NtSLAH1. The open reading frame of NtSLAH1 was 1 107 bp in length and encoded 368 amino acids. The NtSLAH1 protein was located in the cytoplasmic membrane. Comparing to SLAH genes in the other plants, deduced protein sequences of NtSLAH1, AtSLAH1 and AtSLAH4 were highly similar, and NtSLAH1 was closer to AtSLAH1 and AtSLAH4 in evolutionary distance. qRT-PCR results showed that NtSLAH1 was highly expressed in root, and the expression of NtSLAH1, especially in roots, was down-regulated under salt stress. The results indicated that NtSLAH1 might play an important role in response to salt and the absorption of chloride in tobacco.关键词
烟草/慢阴离子通道蛋白/NtSLAH1/氯离子/盐胁迫Key words
Nicotiana tabacum/Slowly activating anion channel protein/NtSLAH1/Chloride ion/Salt stress分类
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张慧,金立锋,徐国云,翟妞,刘萍萍,陈千思,郑庆霞,陈霞,周会娜..烟草慢阴离子通道蛋白基因NtSLAH1的克隆及表达分析[J].烟草科技,2018,51(3):1-6,6.基金项目
中国烟草总公司郑州烟草研究院院长科技发展基金项目"烟草氯离子吸收转运相关基因的筛选及鉴定"(902016CA0190). （902016CA0190）
