眼科新进展2018，Vol.38Issue(3)：201-205,5.DOI:10.13389/j.cnki.rao.2018.0046
TgAPPswePS1转基因小鼠角膜上皮组织病理学和超微结构改变
Changes in histopathology and ultrastructure of corneal epithelium in TgAPPswePS1 transgenic mice
摘要
Abstract
Objective To investigate the histopathological and ultrastructural changes in the corneal epithelium in TgAPPswePS1 transgenic mice.Methods TgAPPswePS1 transgenic mice were randomly divided into experimental group (A and B sub-group) and control group.There were 15 APPswe/PSEN1dE9 transgenic mice (15-18 months old) with Alzheimer's disease (AD) in the experimental A group,and 15 APPswe/PSEN1 dE9 transgenic AD mice (8 months old) mice in the experimental B group,as well as 10 wild-type mice (8 months old) in the control group.Then,the histopathological and ultrastructural changes and the expression of amyloid β-protein (Aβ) in the corneal epithelium of the mice were detected,and finally,the apoptosis of corneal epithelial cells were observed by TUNEL assay.Results The thickness of corneal epithelium in the control group,A and B sub-group of the experimental group was (23.567 ± 2.123) μm,(15.456 ± 1.439) μm and (20.104 ± 1.763) μm,respectively.Meanwhile,murine corneal epithelial cells presented the histopathological changes of disorderly arrangement,decreased layers of cells and irregular morphology in the experimental group compared with the control group.Under transmission electron microscope,the microvilli on the surface of corneal epithelium was flat and significantly decreased in the A and B sub-group when compared with the control group.Moreover,Aβ positive expression in the experimental group was significantly upregulated compared with the control group;and the apoptotic number of the corneal epithelium in the B sub-group [(5.631 ± 2.471) cells] was smaller than that in the A sub-group [(16.329 ±3.542) cells],with the significant difference(P ＜ 0.05).Conclusion There are changes in the histopathology and ultrastructure of the corneal epithelium and the expression of Aβ in TgAPPswePS1 transgenic mice compared with wild-type mice,which are associated with the age of mice.关键词
TgAPPswePS1转基因小鼠/角膜上皮/病理学/超微结构/β淀粉样蛋白Key words
TgAPPswePS1 transgenic mice/corneal epithelium/histopathology/ultrastructure/amyloid β-protein分类
医药卫生引用本文复制引用
罗阿丽,董志章,伍小蝉,李娟,罗阿蓉..TgAPPswePS1转基因小鼠角膜上皮组织病理学和超微结构改变[J].眼科新进展,2018,38(3):201-205,5.基金项目
国家自然科学基金资助(编号:81400424) （编号:81400424）
陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04) （编号:2014K11-03-07-04）
2017年陕西省创新人才推进计划项目-青年科技新星项目(编号:2017KJXX-87)National Natural Science Foundation of China(No:81400424) （编号:2017KJXX-87）
Science and Technology Research and Development Project of Shaanxi Province (No:2014K11-03-07-04) （No:2014K11-03-07-04）
Innovative Talents Promotion Project of Shaanxi Province (No:2017KJXX-87) （No:2017KJXX-87）
