眼科新进展2018，Vol.38Issue(3)：226-229,234,5.DOI:10.13389/j.cnki.rao.2018.0052
载汉防己甲素壳聚糖纳米微球的制备及其对人翼状胬肉细胞增殖的抑制作用
Preparation of chitosan nano-particles loaded tetrandrine and its inhibitory effects on the proliferation of human pterygium fibroblast cells
摘要
Abstract
Objective To investigate the inhibitory effects of chitosan nano-particles loaded tetrandrine (Tet) on proliferation of cultured pterygium fibroblasts.Methods The deoxycholic acid-modified chitosan (DAMC) derivative was synthesized through amidation reaction,and their properties were investigated.The viability of human pterygium fibroblasts (HPF) was evaluated by cell counting kit-8 (CCK-8) assay after cells were interacted with Tet/DAMC nano-particles on day 1,3 and 5.Results The synthesized chitosan derivative and Tet formed drug-loaded nano-particles,and the agent-loading capacity was approximately 76％,and the sizes of agent-loaded nano-particles were 50-500 nm,with Zeta potential values being positive.The result of in vitro drug release experiment indicated that the nano-particles constantly released Tet in a controlled manner within 48 h.The viability of HPF in Tet group was (60.70 ± 2.30) ％,(50.22 ± 2.35) ％,(21.99 ± 2.07) ％ on day 1,3,5,respectively,but the corresponding data in Tet/DAMC group was (79.77 ±2.09)％,(63.24 ±2.83)％,(40.28 ± 1.19)％,respectively.The CCK-8 assay demonstrated that the Tet/DAMC nano-particles could inhibit HPF proliferation,and presented lower toxicity than Tet alone.Conclusion Chitosan nano-particles loaded tetrandrine exhibits a sustained agent-release behavior,which has obvious inhibitory effects on the proliferation of human pterygium fibroblasts,but its cytotoxicity is significantly lower than the original drug's,thereby possessing a great promise for improving the outcome of Tet for the prevention of pterygium recurrence.关键词
汉防己甲素/壳聚糖/纳米微球/翼状胬肉Key words
tetrandrine/chitosan/nano-particle/pterygium分类
医药卫生引用本文复制引用
黄智,周怀胜,许扬扬,胡丹,郑霄,张弛..载汉防己甲素壳聚糖纳米微球的制备及其对人翼状胬肉细胞增殖的抑制作用[J].眼科新进展,2018,38(3):226-229,234,5.基金项目
佛山市科技计划项目(编号:2015AB00272)Science and Technology Foundation of Foshan (No:2015AB00272) （编号:2015AB00272）
