眼科新进展2018，Vol.38Issue(3)：235-238,4.DOI:10.13389/j.cnki.rao.2018.0054
雷帕霉素对高糖诱导人晶状体上皮细胞氧化应激的保护作用
Rapamycin protects human lens epithelial cells against oxidative stress induced by high glucose
齐鹏 1孙丹宇 1包赫 1姜仕先1
作者信息
- 1. 150001 黑龙江省哈尔滨市,哈尔滨医科大学附属第四医院眼视光科
- 折叠
摘要
Abstract
Objective To investigate the effects of rapamycin on human lens epithelial cells (HLECs) against high glucose-induced oxidative stress.Methods A oxidative damage model of HLECs induced by high glucose was established,and intervention with different concentrations of rapamycin for the cells was performed for detecting the survival rate by CCK-8 assay,observing cell morphology by inverted microscope,and measuring the level of intracellular reactive oxygen species (ROS) by H2DCFDA staining,as well as determining the expression of apoptosis related Bcl-2 and Bax proteins by Western blot in all groups.Results CCK-8 results showed that the survival rate of HLECs was increased to 62.00％ ± 3.74％ and 79.57％ ± 5.26％ after treated with 10 nmol · L-1 and 100 nmol · L 1 rapamycin,and the difference was statistically significant when compared with the high glucose group (42.32％ ± 3.10％) (all P ＜ 0.05);H2DCFDA fluorescent probe staining demonstrated that rapamycin could suppress ROS generation in HLECs and alleviate oxidative damage to cells;besides,rapamycin could downregulate the expression of Bcl-2 and Bax protein in HLECs and the inhibitory effects were positively correlated with the concentration of rapamycin.Conclusion Rapamycin can inhibit high glucose-induced oxidative damage and apoptosis m human HLECs,which provides a prevention effect for diabetic cataract.关键词
雷帕霉素/高糖/人晶状体上皮细胞/细胞凋亡Key words
rapamycin/high glucose/human lens epithelium cells/apoptosis分类
医药卫生引用本文复制引用
齐鹏,孙丹宇,包赫,姜仕先..雷帕霉素对高糖诱导人晶状体上皮细胞氧化应激的保护作用[J].眼科新进展,2018,38(3):235-238,4.
