应用生态学报2018，Vol.29Issue(2)：522-530,9.DOI:10.13287/j.1001-9332.201802.026
硝酸盐供应水平对平邑甜茶幼苗生长、光合特性与15N吸收、利用的影响
Effects of nitrate nitrogen supply on the growth, photosynthetic characteristics and 15 N absorption, utilization of Malus hupehensis seedlings
摘要
Abstract
To explore the effects of different nitrate nitrogen levels (N0,N1,N2,N3 and N4 were equivalent to 0,2.5,5,10,20 mmol · L-115 NO3--N,respectively) on the growth,photosynthetic characteristics and 15N absorption,utilization and distribution,Malus hupehensis seedlings were grown in cultural liquid Hoagland by using the 15N-labeled tracer method.The results showed that the leaf chlorophyll content,leaf area and dry mass in different organs were the highest in N2 treatment.With the increase of 15NO3--N application rates,the leaf net photosynthetic rate (Pn)significantly increased but tended to decease when the 15NO3--N concentration exceeded N2 treatment.In the 20th day after treatment,the root activity,root length,root surface area and number of tips of seedlings in N2 treatment were significantly higher than those in the other treatments.The distribution ratio of 15N in different organs was significantly different among those treatments.The relatively balanced distribution ratio of 15N appeared in N2 treatment,which the 15N utilization rate also reached relatively higher level.The total N content and 15N absorption of seedlings increased at low 15NO3--N concentration,reached the highest value in N2 treatment with 103.77 and 21.57 mg,and then deceased at high 15NO3--N concentration.At the 12th day after treatment,the leaf nitrate reductase (NR) activity was the highest in N2 treatment and the lowest in N4 treatment.The leaf nitrate reductase (NR) activity deceased by 84.9％ in N4 treatment compared with N2 treatment at the 16th day after treatment.Our findings indicated that the photosynthesis and absorption of nitrate nitrogen were inhibited under low 15NO3--N stress,and the assimilation of nitrate nitrogen and root growth were restrained under too much higher 15NO3--N level,which was not good for the growth,nitrogen absorption and utilization of apple seedlings.The appropriate nitrogen level could promote plant growth,enhance the photosynthesis and also increase the absorption,utilization and distribution of nitrogen.关键词
苹果砧木/硝态氮水平/光合/15NKey words
apple rootstock/nitrate nitrogen level/photosynthesis/15N.引用本文复制引用
彭玲,刘晶晶,王芬,葛顺峰,姜远茂..硝酸盐供应水平对平邑甜茶幼苗生长、光合特性与15N吸收、利用的影响[J].应用生态学报,2018,29(2):522-530,9.基金项目
本文由国家自然科学基金项目(31501713)、国家重点研发计划项目(2016YFD0201100)、国家现代农业产业技术体系建设资金项目(CARS-28)和山东省自然科学基金项目(ZR2015PC001)资助 This work was supported by the National Natural Science Foundation of China (31501713),the National Key Research and Development Program of China (2016YFD0201100),the China Modem Agriculture Industry System Construction Foundation (CARS-28),and the Natural Science Foundation of Shandong Province,China (ZR2015PC001). （31501713）
