应用生态学报2018，Vol.29Issue(2)：554-564,11.DOI:10.13287/j.1001-9332.201802.021
干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制
Physiological and biochemical mechanism of spermidine improving drought resistance in maize seedlings under drought stress
摘要
Abstract
To explore the role of exogenous spermidine (Spd) in enhancing the resistance of maize to drought stress,15％ polyethylene glycol (PEG-6000) was used to simulate drought stress and with 'Xianyu 335'(drought-insensitive) and'Fenghe 1'(drought sensitive) as the experiment materials,the effects of Spd (0.1 mmol · L-1) on the growth,photosynthetic characteristics,chlorophyll content,osmotic adjustment substance,membrane lipid peroxidation and root activity of maize seedlings were investigated.The results showed that the application of Spd significantly promoted the growth of maize seedlings under drought stress,increased chlorophyll content,net photosynthetic rate (Pn),stomatal conductance (gs),transpiration rate (Tr) and water use efficiency (WUE),and decreased the enhancement of intercellular carbon dioxide concentration (Ci) in 'Fenghe 1'.Moreover,the stomatal and non-stomatal limitations of photosynthetic ability caused by drought stress in'Fenghe 1'were effectively reduced by exogenous Spd.The application of Spd increased the content of proline and soluble sugar,decreased the O2 + generation rate,contents of H2O2 and MDA and cell membrane permeability,enhanced the root activity.The changes of drought-sensitive'Fenghe 1'were greater than drought-tolerant'Xianyu 335'.These results indicated that exogenous Spd had positive effects on the seedlings to capture and converse solar energy,thus promoting photosynthesis and the growth of maize seedlings.It would also enhance the adaptability of seedlings to drought stress by reducing the production of reactive oxygen species (ROS),increasing the accumulation of osmotic adjustment substances to stabilize the cell membrane system and improving the root vigor.The positive effects of Spd was more obvious for drought-sensitive variety'Fenghe 1'.关键词
玉米幼苗/亚精胺/干旱胁迫/光合特性/渗透调节/细胞膜透性Key words
maize seedling/spermidine/drought stress/photosynthetic characteristics/osmotic adjustment/cell membrane permeability引用本文复制引用
李丽杰,顾万荣,孟瑶,王悦力,穆君怡,李晶,魏湜..干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制[J].应用生态学报,2018,29(2):554-564,11.基金项目
本文由国家重点研发计划项目(2016YFD0300103)和东北农业大学“学术骨干”(17XG23)项目资助 This work was supported by the National Key Research and Development Project (2016YFD0300103) and the Academic Backbone Project of Northeast Agricultural University (17XG23). （2016YFD0300103）
