应用生态学报2018，Vol.29Issue(2)：659-668,10.DOI:10.13287/j.1001-9332.201802.032
黑腹果蝇white基因在H2O2影响下卷翅形成中的作用及机理
The role of white gene and its mechanism during the formation of curly wing in Drosophila melanogaster
摘要
Abstract
Curly wing is one of the most frequently used genetic markers in Drosophila melanogaster,but its molecular mechanism is remained unclear.Previous results have showed that physicochemical stimulation would affect the formation of the cruly wing.Our recent study found that H2O2 could not only affect the eclosion rate of D.melanogaster,but also induce the formation of curly wing.Here,we aimed to uncover the specific factors influencing the formation of curly wing in D.melanogaster via changing the concentration of H2O2 and the temperature as well as the time of H2O2 treatment.We measured the activities of superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-PX),in order to examine the effects of H2O2 on antioxidative capacity of D.melanogaster.The results showed that the eclosion rate of D.melanogaster was inversely correlated with the concentration of H2O2.The change of temperature,H2O2 concentration and the period of H2O2 treatment affected the degree of the curl and the proportion of the curly wing.The white mutant flies responded most significantly to these three conditions,the mini-white (white gene reverse mutation) flies could rescue the curly phenotype,and responded similarly to the wild type OR.H2O2 had effects on the formation of the curly wing which contained the Cy mutation,leading to increased rate of the curly wing.D.melanogaster treated with H2O2 would reduce the antioxidative capacity.Results from real-time quantitative PCR showed that H2O2 treatment resulted in a change in gene expression.The formation of curly wing was a complicated process,and H2O2 might act as a signaling molecule or indirectly affect certain factors in the formation of curly wing in D.melanogaster.This process might share the same signaling pathway with the Cy mutant,or might be regulated by different modulating patterns.关键词
white基因/黑腹果蝇/卷翅/H2O2/氧化Key words
white gene/Drosophila melanogaster/curly wing/H2O2/oxidation引用本文复制引用
吴程丽,林欣大,蒋艳云,孟帅帅..黑腹果蝇white基因在H2O2影响下卷翅形成中的作用及机理[J].应用生态学报,2018,29(2):659-668,10.基金项目
本文由国家自然科学基金项目(31071297)资助 This work was supported by the National Natural Science Foundation of China (31071297). （31071297）
