中国动物检疫2018，Vol.35Issue(4)：106-110,5.DOI:10.3969/j.issn.1005-944X.2018.04.026
不同结构羊舍空气可培养真菌ERIC-PCR指纹图谱分析
ERIC-PCR Fingerprinting Analysis of Airborne Fungi in Sheep Sheds with Different Structures
摘要
Abstract
In order to investigate the structure of airborne fungi in sheep sheds,so as to provide basis for sheep shed selection and environment control. AGI-30 sampler was used to collect the ultrapure water as the medium,and airborne fungi from 2 kinds of sheep sheds(including structures of ground raising and high-bed raising)were collected. After enrichment cultivation with potato dextrose broth,the fungal genomic DNA was extracted and the diversity of airborne fungi was analyzed by ERIC-PCR. The results showed that there were differences in the structure of airborne fungal flora in the two kinds of sheep sheds,and there were some similar dominant bacteria flora. The diversity index in the sheds with high beds was higher than that of the ground sheep sheds,but the dominance index was low. Similarity clustering analysis showed that the structure of airborne fungi in the sheds with same structure was stable in the same season,and it changed greatly in different seasons. As a conclusion,the diversity of airborne fungi was variable in sheep sheds and was affected by both sheep shed structures and seasons.关键词
羊舍/结构/可培养气载真菌/ERIC-PCRKey words
sheep shed/structure/culturable airborne fungi/ERIC-PCR分类
农业科技引用本文复制引用
乔婷婷,刘兴国,古少鹏,于欢,段晋伟,刘家祺,白燕雨,郭耀,高晓莎,张艳冰,霍乃蕊..不同结构羊舍空气可培养真菌ERIC-PCR指纹图谱分析[J].中国动物检疫,2018,35(4):106-110,5.基金项目
国家自然基金(31201437) （31201437）
中国动物疫病预防控制中心横向课题(2013HX45) （2013HX45）
山西省农业厅横向课题(2013HX35) （2013HX35）
