中国康复理论与实践2018，Vol.24Issue(3)：277-281,5.DOI:10.3969/j.issn.1006-9771.2018.03.005
加味脑泰方对卵巢摘除脑缺血大鼠海马细胞外信号调节蛋白激酶1/2和c-Jun氨基末端蛋白激酶蛋白活化的影响
Effect of Jiawei Naotaifang on Activation of Extracellular Signal-regulated Kinase 1/2 and c-Jun N-terminal Kinase in Ovariectomized Rats after Cerebral Ischemia
摘要
Abstract
Objective To investigate the effect of Jiawei Naotaifang on neuronal apoptosis and the mechanism in ovariectomized rats with cerebral ischemia. Methods Female Sprague-Dawley rats(n=40)were randomly divided into sham group(n=10),model group(n=10),es-trogen group(n=10)and Jiawei Naotaifang group(n=10).The model group,estrogen group and Jiawei Naotai-fang group were ovariectomized.Eleven days after ovariectomy,the estrogen group and Jiawei Naotaifang group were given estrogen and Jiawei Naotaifang respectively intragastrically for three days.14 days after ovariecto-my,the model group,estrogen group and Jiawei Naotaifang group were modeled cerebral ischemia with Langa's method.24 hours after modeling,the apoptosis rate of neurons was detected with TUNEL,and the activation of extracellular signal-regulated kinase 1/2(ERK1/2)and c-Jun N-terminal kinase(p-JNK)in hippocampus were de-tected with Western blotting. Results Compared with the model group, the apoptosis rates decreased in Jiawei Naotaifang group and the estrogen group(P<0.001),with more activation of ERK1/2(P<0.01)and less activation of JNK(P<0.01). Conclusion Jiawei Naotaifang can protect neuron from apoptosis by promoting the activation of ERK1/2 and inhibiting the activation of p-JNK.关键词
脑缺血/加味脑泰方/中药/凋亡/细胞外信号调节蛋白激酶1/2/磷酸化c-Jun氨基末端蛋白激酶/大鼠Key words
cerebral ischemia/Jiawei Naotaifang/traditional Chinese medicine/apoptosis/extracellular signal-regulat-ed kinase 1/2/c-Jun N-terminal kinase/rats分类
医药卫生引用本文复制引用
秦莉花,李晟,成邵武,刘林,刘洋,黄娟,龚胜强,程诚,葛金文..加味脑泰方对卵巢摘除脑缺血大鼠海马细胞外信号调节蛋白激酶1/2和c-Jun氨基末端蛋白激酶蛋白活化的影响[J].中国康复理论与实践,2018,24(3):277-281,5.基金项目
湖南省中医药科研计划一般项目(No.201784) （No.201784）
湖南省教育厅优秀青年科研项目(No.17B196) （No.17B196）
湖南省教育厅一般科研项目(No.14C0857)Hunan Research Projects of Chinese Traditional Medicine(General)(No.201784),Hunan Education Department Research Project(Youth)(No.17B196)and Hunan Education Department Research Project(General)(No.14C0857) （No.14C0857）
