中国普通外科杂志2018，Vol.27Issue(3)：321-327,7.DOI:10.3978/j.issn.1005-6947.2018.03.009
下调G蛋白信号调控因子2表达对胰腺癌细胞化疗敏感性的影响
Effect of down-regulating G protein signal regulating protein2 expression on chemotherapy sensitivity of pancreatic cancer cells
摘要
Abstract
Objective: To investigate the effect of down-regulating G protein signal regulating protein 2 (GPSM2) expression on chemotherapy sensitivity of pancreatic cancer cells. Methods: hTe pancreatic cancer MIA-PaCa-2 cells with low GPSM2 expression were constructed and identiifed.Sixteen nude mice were equally randomized into two groups, and were subcutaneously implanted with MIA-PaCa-2 cells with low GPSM2 expression and MIA-PaCa-2 cells with natural GPSM2 expression to establish the tumor-bearing models. Atfer that, half mice underwent gemcitabine injection (100 mg/kg) and half mice were given saline of the same volume in each group (intraperitoneal injection, 3 times per week for 4 weeks). hTe tumor growth curves were drawn, and all mice were sacriifced on the third day atfer the last injection, and the volumes of the tumor xenogratfs were determined. Results: hTe pancreatic cancer MIA-PaCa-2 cells with low GPSM2 expression were successfully created. Both growth speed and volume of the tumor xenogratfs presented a decreasing trend as natural GPSM2 expression plus saline group>natural GPSM2 expression plus gemcitabine group>low GPSM2 expression plus saline group>low GPSM2 expression plus gemcitabine group. hTe result of factorial design showed that the main effect of either gemcitabine alone or down-regulating GPSM2 gene expression alone on tumor growth inhibition had statistical signiifcance (bothP=0.000), while the interaction effect of gemcitabine combined with down-regulating GPSM2 gene expression on tumor growth inhibition did not reach a statistical significance (P=0.073), but the simple effect of gemcitabine combined with down-regulating GPSM2 gene expression on tumor growth inhibition had statistical signiifcance compared with gemcitabine alone or down-regulating GPSM2 gene expression alone (P=0.000 and 0.003). Conclusion: Whether down-regulating GPSM2 gene expression will enhance chemotherapy sensitivity of pancreatic cancer cells cannot be confirmed. However, the inhibitory effect of down-regulating GPSM2 gene expression with simultaneous chemotherapy on pancreatic cancer is remarkably greater than that of their single actions.关键词
胰腺肿瘤/RGS蛋白质类/化疗/异种移植模型抗肿瘤试验Key words
Pancreatic Neoplasms/RGS Proteins/Chemotherapy/Xenogratf Model Antitumor Assays分类
医药卫生引用本文复制引用
郑伟,张建新,谭克,陈思澈,范逸怡,党胜春..下调G蛋白信号调控因子2表达对胰腺癌细胞化疗敏感性的影响[J].中国普通外科杂志,2018,27(3):321-327,7.基金项目
江苏省六大高峰人才计划资助项目(WSN-007). （WSN-007）
