中国全科医学2018，Vol.21Issue(11)：1310-1314,5.DOI:10.3969/j.issn.1007-9572.2018.11.010
停灌与再灌注时间对心肌缺血再灌注损伤程度的影响研究
Effect of Suspension Time and Reperfusion Time on Myocardial Ischemia-reperfusion Injury
摘要
Abstract
Objective To investigate the effect of suspension time and perfusion time on myocardial ischemia-reperfusion injury,in order to provide experimental evidence for the prevention or reduction of ischemia-reperfusion injury. Methods This study was conducted between September 2015 and May 2017.Healthy adult male tree shrews were divided into five groups by random number table,and there were 10 tree shrews in each group,which were successfully established myocardial ischemia-reperfusion injury models through Langendorff technique.Treatment in group A was stable perfusion for 30 min,and 5 tree shrews were uesd to observed infarction areas,and the others were continue perfusing for 60 min.In group B,treatment included stable perfusion for 30 min,suspension for 15 min and reperfusion for 30 min;in group C,treatment was stable perfusion for 30 min,suspension for 15 min and reperfusion for 60 min.In group D,stable perfusion,suspension and reperfusion were undertaken for 30,30 and 30 min,respectively;in group E,stable perfusion,suspension and reperfusion were undertaken for 30,30 and 60 min,respectively.In B-E groups,5 tree shrews were uesd to observed infarction areas after suspension,and the others were continue reperfusing.The infarction area was calculated based on heart coronal sections.Perfusion fluid and myocardial tissues were obtained to determine the levels of alanine aminotransferase(ALT),aspartate transaminase (AST),creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH).Results There was no statistically significant difference in myocardial infarction area between suspension and reperfusion in group A,D and E(P>0.05).The myocardial infarction area were increased after reperfusion than that after suspension in group B and C(P<0.05).The infarction areas in group B-E were significantly larger than that in group A,and group D and E had a larger infarction area than group B and C (P<0.05).Two-factor analysis of variance showed that the main effect of suspension time was significant for the ALT and AST level in perfusion fluid and myocardial tissues,as well as for the level of CK-MB and LDH in perfusion fluid;while the main effect of reperfusion time was significant for the ALT,AST,CK-MB and LDH levels in both perfusion fluid and myocardial tissues(P<0.05).Conclusion Both suspension time and reperfusion time are key factors in affecting myocardial ischemia-reperfusion injury,and reperfusion time is more important.关键词
心肌再灌注损伤/停灌时间/再灌注时间Key words
Myocardial reperfusion injury/Ischemia time/Reperfusion time分类
医药卫生引用本文复制引用
杨天睿,苗云波,张彤,段靳岚,朱滢,穆宁晖,余锦雯..停灌与再灌注时间对心肌缺血再灌注损伤程度的影响研究[J].中国全科医学,2018,21(11):1310-1314,5.基金项目
云南省内设研究机构老年病防治研究中心立项课题(2016NS203) （2016NS203）
云南省卫计委医学后备人才培养计划(H-201615) （H-201615）
云南省应用基础研究〔昆医联合专项,2017FE468(-112)〕 （-112）
云南省应用基础研究〔昆医联合专项,2017FE467(-109)〕 （-109）
