中国全科医学2018，Vol.21Issue(11)：1315-1320,6.DOI:10.3969/j.issn.1007-9572.2018.00.052
湿润烧伤膏对糖尿病大鼠创面组织细胞间黏附分子1和血管细胞黏附分子1 mRNA表达水平及超微结构的影响研究
Effect of MEBO on ICAM-1 mRNA and VCAM-1 mRNA Expression Levels and Ultrastructure in Wound Tissue of Rats with Diabetic Ulcer
摘要
Abstract
Objective To explore the effect and mechanism of MEBO on ICAM-1 mRNA,VCAM-1 mRNA expression levels and ultrastructure in wound tissue of rats with diabetic ulcer.Methods A total of 145 12-week-old healthy male SD rats of SPF grade were randomly assigned to blank group (n=35) and diabetic model group (n=110) by the random digital table in June 2015.Diabetic model was induced by intraperitoneal injection of streptozotocin(STZ).Eight weeks later, 30 rats in blank group and 90 diabetic rats (equally divided into model subgroup,bFGF subgroup and MEBO subgroup) were selected for establishing diabetic ulcer models.Then,both the blank group and model subgroup,bFGF subgroup,and MEBO subgroup received local treatment of the ulcer with normal saline-moistened gauze,bFGF-moistened gauze,and MEBO-moistened gauze,respectively.The dressing was changed once a day for 12 days.At 3,6 and 12 days after intervention,10 rats were selected randomly from each group for taking the wound tissue from the same site for test.The expression levels of ICAM-1 mRNA and VCAM-1 mRNA were measured by fluorescent PCR and the ultrastructural pathology of the wound tissue was observed by electron microscopy.Results Compared with the blank group,the mean expression level of ICAM-1 mRNA in the model subgroup was lower at 3 days after intervention,but higher at 6 and 12 days after intervention (P<0.05).The expression level of ICAM-1 mRNA in the bFGF subgroup was significantly lower than model subgroup at 12 days after intervention(P<0.05).The expression level of ICAM-1 mRNA in the MEBO subgroup was higher than that in the model subgroup at 3 days after intervention (P<0.05), but it was lower than that in the model subgroup at 6 and 12 day s after intervention (P<0.05).Compared with the model subgroup,blank group presented higher mean VCAM-1 mRNA expression level at 3 days after intervention,but lower mean VCAM-1 mRNA expression level at 6,12 days after intervention(P<0.05),as did both bFGF and MEBO subgroups (P<0.05).Pathological results showed that at 6 and 12 days after intervention,in both MEBO and bFGF subgroups,the cell structure of the wound tissue was improved gradually,contributing to the recovery of organelle morphology,while in the model subgroup,similar status was not found.Conclusion MEBO could obviously promote the healing of diabetic ulcer by regulating the expression of downstream factors ICAM-1 and VCAM-1 mRNA in AGEs-RAGE signaling pathway and improving cell ultrastructure in wound tissue.关键词
糖尿病/溃疡/湿润烧伤膏/细胞间黏附分子1/血管细胞黏附分子1/超微病理学Key words
Diabetes mellitus/Ulcer/MEBO/Intercellular adhesion molecule-1/Vascular cell adhesion molecule-1/Ultrastructural pathology分类
医药卫生引用本文复制引用
王丽,李杰辉,张春霞,狄钾骐,刘明,刘雪琴..湿润烧伤膏对糖尿病大鼠创面组织细胞间黏附分子1和血管细胞黏附分子1 mRNA表达水平及超微结构的影响研究[J].中国全科医学,2018,21(11):1315-1320,6.基金项目
国家自然科学基金资助项目(81660793) （81660793）
国家自然科学基金委员会青年科学基金项目(81302975) （81302975）
广西科技重点研发计划项目(桂科AB17292046) （桂科AB17292046）
