中国人兽共患病学报2018，Vol.34Issue(3)：255-259,5.DOI:10.3969/j.issn.1002-2694.2018.00.032
云南省永善县恙虫病流行病学调查
Investigation on scrub typhus in Yongshan County,Yunnan Province,China
摘要
Abstract
We investigated epidemiological characteristic of scrub typhus in Yongshan County,Yunnan Province,China. The serum samples were collected from the patients with fever for detecting the antibody against Orientia tsutsugamsushi (Ot) by colloidal gold immunoassay assay.Rat traps were used to capture rodents.The spleen tissues of the captured rodents were detected by nested-polymerase chain reaction for rickettsia groEL segment.The groEL segments were sequenced and analyzed the homology with the other known sequences.Thirty-four scrub typhus cases were found in Yongshan County,Yunnan Prov-ince from May 2015 to October 2015.Among them,21 cases were confirmed by laboratory tests and 13 cases were clinical di-agnosis diseases.Of these patients,32.35% of the cases occurred in June.The 32.35% were in the group of the 40-49 year-old,and 79.41% were farmers,94.12% exhibited eschar or skin ulcer(31.25% were observed in groin of these cases),and rash developed in 50%.In 39 spleen tissue samples of Rattus flavipectus,9 samples showed positive for groEL gene Ot,but gro-EL gene of Typhus group rickettsia and spotted fever group rickettsia were negative.Sequence analysis showed that YSP30 was closely related to some Saitama related strains of Ot,such as HSB1,FAR1 and UAP4,while the other 8 strains were closely related to some Karp related strains of Ot,such as UT213,UT221 and SH205.It was confirmed that the Yongshan County was the natural foci of scrub typhus by the serological and molecular biological detections.There are Karp and Saitama genotype related Ots in the natural foci.关键词
恙虫病/恙虫病东方体/流行病学/基因序列分析/永善县Key words
scrub typhus/Orientia tsutsugamushi/epi-demiology/gene sequence analysis/Yongshan County分类
医药卫生引用本文复制引用
亚红祥,董万贤,魏培发,周天惠,周济华,张云智,樊荣..云南省永善县恙虫病流行病学调查[J].中国人兽共患病学报,2018,34(3):255-259,5.基金项目
国家自然科学基金(No.81660558,No.81260437) （No.81660558,No.81260437）
科技基础性工作专项重点项目(No.2013FY113500) （No.2013FY113500）
云南公共卫生与疾病防控协同创新中心项目(No.2015YNPHXT05)Supported by the National Natural Science Foundation of China (Nos.81660558 and 81260437),the Scientific and Technologi-cal Basis Special Project from the Minister of Science and Technology of the People's Republic of China (No.2013FY113500) and the Project of Yunnan Provincial Collaborative Innovation Center for Public Health and Disease Prevention and Control(No. 2015YNPHXT05) （No.2015YNPHXT05）
