中国人兽共患病学报2018，Vol.34Issue(3)：260-266,7.DOI:10.3969/j.issn.1002-2694.2018.00.041
用CRISPR/Cas9系统消除潜伏的HIV前病毒基因组及其感染细胞的研究进展
Elimination of latent HIV genome and its infected cells by CRISPR/Cas9 system
摘要
Abstract
The incidence and mortality of AIDS have been decreasing after the adoption of combined antiretroviral therapy strategy in the world,then AIDS has become a manageable chronic infectious disease.But HIV/AIDS continues to be a major global public health problem since it is restricted by a variety of factors.The major reason for the persistence of HIV/AIDS is the inability of existing treatments to clear or eradicate the multiple HIV reservoirs that exist in the human body.To suppress the virus replication and rebound,HIV/AIDS patients must take life-long antiviral medications.A few years ago,the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9)system has been developed as a simple,fast and easy to operate gene-editing technique.Several studies in HIV infected cells and/or in animal models have shown that the system has the potential to eliminate or disrupt HIV-integrated genome or HIV-infected cells from multiple HIV reservoirs,which may result in the complete cure of HIV/AIDS.This paper analyzes the results of CRISPR/CAS9 in the elimination of latent HIV,and discusses the possible problems and trends.关键词
CRISPR Cas9系统/基因编辑技术/潜伏HIV基因组和细胞Key words
CRISPR/Cas9 system/gene-editing technique/latent HIV genome and cells分类
医药卫生引用本文复制引用
严延生,吴守丽..用CRISPR/Cas9系统消除潜伏的HIV前病毒基因组及其感染细胞的研究进展[J].中国人兽共患病学报,2018,34(3):260-266,7.基金项目
闽科计 [2016]9号社会发展引导性 (重点)项目资助 (No. 2016Y0010)和福建省卫生系统中青年骨干人才培养项目(No.2015-ZQNZD-11)联合资助Supported by the Guiding Project for Fujian Social Development(No.2016Y0010)and the Training Project for Fujian Provincial Health System for Young and Middle-aged Backbone Personnels(No.2015-ZQNZD-11) （重点）
