中国蔬菜Issue(4)：30-36,7.
结球甘蓝小孢子培养条件优化及高代自交系胚状体诱导研究
Studies on Optimization of Cabbage Isolated Microspore Culture Conditions and Generation of Embryoids from High-generation Inbred Lines
摘要
Abstract
Taking 25 different types of cabbage(Brassica oleracea L. var. capitata L.)accessions as material,this paper studied on the factors affecting the generation of embryoids in culturing isolated microspore and conducted optimization.The embryogenesis for high-generation inbred lines was studied as well.The results showed that the donor plant genotype is the key factor affecting embryogenesis.Embryoids were induced from 11 accessions among all the tested materials.The highest embryoid inducing rate was 19.8 per bud in the hybrid'Zhonggan 628'and 47.5 per bud in inbred line'01-88',which were extremely significantly higher than that of the other accessions.Floral buds with anther length between 3.0-3.5 mm,and ratio of anther length to petal length of 3:2-2:1,could produce the highest proportion of microspores at late-uninucleate stage.Early and florescence stages were the optimum sampling periods.Adding active carbon could extremely significantly improve embryoid induction rate.关键词
结球甘蓝/游离小孢子培养/条件优化/高代自交系/胚状体诱导Key words
Cabbage/Isolated microspore culture/Condition optimization/High-generation inbred lines/Embryo induction引用本文复制引用
苏贺楠,韩风庆,杨丽梅,庄木,张扬勇,王勇,李占省,方智远,吕红豪..结球甘蓝小孢子培养条件优化及高代自交系胚状体诱导研究[J].中国蔬菜,2018,(4):30-36,7.基金项目
国家重点研发计划项目(2017YFD0101804,2016YFD0100204),中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS),大宗蔬菜产业技术体系项目(nycytx-35-gw01) （2017YFD0101804,2016YFD0100204）
