中国现代医学杂志2018，Vol.28Issue(10)：26-31,6.DOI:10.3969/j.issn.1005-8982.2018.10.005
MicroRNA-195参与切应力对血管内皮细胞炎症调控作用的研究
Role of miR-195 in regulation of vascular endothelial cell inflammation by shear stress
吴小苑 1范文冬2
作者信息
- 1. 广东省职业病防治院 广东 广州 510300
- 2. 中山大学附属第一医院心内科,广东 广州 510080
- 折叠
摘要
Abstract
Objective To investigate whether miR-195 is involved in the regulation of vascular endothelial cell (EC) inflammation by shear stress. Methods To verify the regulation of EC miRNA expression by physiological shear stress, 15 dyne/cm2of physiological shear stress were used to process human umbilical vein endothelial cells (HUVECs), after 24 hours, the expressions of miRNAs (miR-27b, miR-143/145, miR-195, etc.) in endothelial cells were detected by qRT-PCR. To study the relationship between miR-195 and endothelial cell inflammatory response, miR-195 mimics and inhibitors were transfected into HUVECs in vitro,the expressions of inflammatory factors in ECs and the effect of miR-195 in controlling endothelial cell inflammation were detected using Western blot and immunofluorescence technique. Results After processing HUVECs with 15 dyne/cm2of physiological shear stress for 24 hours, the expressions of miR-27b, miR-143/145 and miR-195 were significantly up-regulated (P < 0.05). The over-expression of miR-195 did not affect the expression of eNOS, but significantly inhibited the expression of P-p65(P < 0.05); while inhibition of endogenous miR-195 by miRNA inhibitors significantly reduced the expression of EC eNOS (P < 0.05) and inhibited the expression of IKBa (P < 0.05). Immunofluorescence results showed that over-expression of miR-195 in ECs inhibited nuclear translocation of NF-κB, while inhibition of miR-195 may promote the nuclear translocation of NF-κB and promote inflammation. Conclusions The expression of miR-195 is significantly up-regulated by physiological shear stress. miR-195 may be involved in the regulation of EC inflammation by shear stress.关键词
miR-195/内皮细胞/炎症反应Key words
miR-195/endothelial cell/inflammatory response分类
医药卫生引用本文复制引用
吴小苑,范文冬..MicroRNA-195参与切应力对血管内皮细胞炎症调控作用的研究[J].中国现代医学杂志,2018,28(10):26-31,6.
