中国现代医学杂志2018，Vol.28Issue(10)：32-36,5.DOI:10.3969/j.issn.1005-8982.2018.10.006
耐甲氧西林金黄色葡萄球菌MLST联合SCCmec基因分型与耐药性分析
Drug resistance and MLST combined with SCCmec genotyping of Methicillin-resistant Staphylococcus aureus
摘要
Abstract
Objective To investigate the drug resistance and genetic background of Methicillin-resistant Staphylococcus aureus(MRSA). Methods Multilocus sequence typing(MLST)technique was introduced to identify sequence types (ST) of MRSA, SCCmec types were determined by multiplex PCR. MLST plus SCCmec typing of these strains were used for their genotyping at the same time. The susceptibility to antimicrobial agents was tested by micro-dilution method. Results Among the 67 isolates of MRSA, 63 (94.03%) isolates belonged to SCCmec type III;6 different genotypes were detected by MLST typing which included ST88, ST121, ST221, ST82, ST239 and ST399, and 61 (91.04%) isolates belonged to ST239. Drug resistance analysis showed the MRSA strains of SCCmec type III were multi-resistant. Conclusions ST239-MRSA-SCCmec III genotype is the major epidemic strain in northern Shangdong Province, which has multiple resistance to antibiotics. MLST combined with SCCmec genotyping is a rapid and simple method of MRSA genotyping.关键词
金黄色葡萄球菌/多位点序列分型/葡萄球菌mec盒式染色体/耐药Key words
Staphylococcus aureus/Methicillin/multilocus sequence typing(MLST)/Staphylococcal cassette chromosome mec (SCCmec)/resistance分类
医药卫生引用本文复制引用
纪冰,王凯凯,赵红梅,董艳,孟玮,邢敏,孙吉花..耐甲氧西林金黄色葡萄球菌MLST联合SCCmec基因分型与耐药性分析[J].中国现代医学杂志,2018,28(10):32-36,5.基金项目
滨州市科技发展计划项目(No:2013ZC1711) （No:2013ZC1711）
滨州医学院科技计划(No:BY2013KJ02) （No:BY2013KJ02）
山东省医药卫生科技发展计划(No:2015WS0491,No:2017WS372) （No:2015WS0491,No:2017WS372）
