中国现代医学杂志2018，Vol.28Issue(10)：37-42,6.DOI:10.3969/j.issn.1005-8982.2018.10.007
江西及周边地区神经系统疾病患者博尔纳病毒感染的分子流行病学研究
Molecular epidemiology of Borna virus infection in patients with nervous diseases in Jiangxi and its surrounding areas
摘要
Abstract
Objective To investigate the epidemic situation of Borna virus (BDV) infection in Jiangxi and the surrounding areas. Methods Nested reverse transcriptase polymerase chain reaction (nRT-PCR) combined with fluorescence quantitative PCR (qRT-PCR) was used to detect BDV p24 gene fragment of the peripheral blood mononuclear cells (PBMCs) in 30 patients of viral encephalitis (VE), 10 patients of multiple sclerosis (MS), 10 patients of acute Guillain Barre syndrome (GBS), 10 patients with Parkinson's disease (PD), 60 healthy people, and 60 goats. Keegan sequence and homology analysis of the positive products were carried out, and the characteristics of BDV molecular epidemiology were analyzed. Results The positive rate of BDV p24 gene fragment in the VE patients and the MS patients were significantly higher than that in the healthy subjects (P < 0.05), while the BDV p24 gene fragment positive rate in the goats was 1.67%. The sequence of BDV p24 gene fragment was consistent in the VE patients and the MS patients, and the homology with strain V was 95.73%, and the homology with BDV/MDCK strain was 97.89%. When the results of BDV p24 gene sequencing in the goats were compared with that of strain V provided by GenBank, the homology was 95.72%; when compared with that of BDV/MDCK strain, the homology was 96.38%; when compared with C6BV strain, the homology was 96.49%, but there was no substantial change in the amino acid encoded by the strain. Conclusions BDV may be a new pathogen of infectious diseases, and may be closely correlated with the occurrence of some neurological diseases (such as VE, MS) in human. There may be animal origin of BDV infection in some patients with nervous system diseases in Jiangxi and its surrounding areas. It needs further in-depth research and analysis, so as to improve the understanding of this kind of disease, and to prevent and control it more effectively.关键词
神经疾病/博尔纳病毒/感染/分子流行病学Key words
neurological disease/Borna virus/infection/molecular epidemiology分类
医药卫生引用本文复制引用
吴伟,谢洪武,谢亮,黄孝天,谢鹏..江西及周边地区神经系统疾病患者博尔纳病毒感染的分子流行病学研究[J].中国现代医学杂志,2018,28(10):37-42,6.基金项目
国家自然科学基金(No:30470605) （No:30470605）
江西省卫生厅科技计划(No:20073069) （No:20073069）
