中国药房2018，Vol.29Issue(6)：753-756,4.DOI:10.6039/j.issn.1001-0408.2018.06.08
多指标评分法优选野马追抗动脉粥样硬化有效部位的乙醇提取工艺
Optimization of Eehanol Extraction Technology of Anti-atherosclerosis Effective Fraction in Lindley eupatorium by Multiple Index Scoring Method
摘要
Abstract
OBJECTIVE:To optimize the ethanol extraction process of anti-atherosclerosis effective fraction of Lindley eupatorium. METHODS:Using the contents of hyperin,quercetin,kaempferol and jaceosidin as indexes,the effects of ethanol volume fraction,liquid-solid ratio,extraction time and extraction times on ethanol extraction technology were investigated. By multiple index scoring method,orthogonal test was designed to optimize extraction technology,and validation test was conducted. RESULTS:The optimal extraction technology was 70% ethanol as extraction solvent with solid-liquid ratio of 12 : 1,reflux extracting for 3 times,2 h each time. Results of 3 batches of validation tests showed that the contents of 4 marker components were in high level,being 5.305 3,1.560 1,1.986 5,6.703 6 mg/g in average(RSD<1.1%,n=3). Results of validation tests were stable and reliable,and were in good agreement with the results of orthogonal tests. CONCLUSIONS:The optimized ethanol reflux extraction technology of L. eupatorium is stable and reliable,and performs well. Energy consumption and cost are suitable for industrial production.The results provide technology basis for further purification and enrichment of anti-atherosclerosis effective components of L.eupatorium.关键词
野马追/抗动脉粥样硬化/提取工艺/正交试验/多指标评分法Key words
Lindley eupatorium/Anti-atherosclerosis/Extraction technology/Orthogonal test/Multiple index scoring method分类
医药卫生引用本文复制引用
李卿,贺宗毅,杜洪飞,周兴,梁旭明..多指标评分法优选野马追抗动脉粥样硬化有效部位的乙醇提取工艺[J].中国药房,2018,29(6):753-756,4.基金项目
重庆市重点产业共性关键技术创新专项(No. cstc2016zdcy-ztzx10004) （No. cstc2016zdcy-ztzx10004）
重庆市基本科研业务费计划项目(No.2016cstc-jbky-01904) （No.2016cstc-jbky-01904）
